Cellular characterization of V cell populations (novel antigen specific immunoglobulin expressing cell lineage) from immunized mice (P4402)
| العنوان: | Cellular characterization of V cell populations (novel antigen specific immunoglobulin expressing cell lineage) from immunized mice (P4402) |
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| المؤلفون: | Jeanne Elia, Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Jacob Rabenstein, Andrea Nguyen, Janean Fisher, Jody Berry |
| المصدر: | The Journal of Immunology. 190:52.21-52.21 |
| بيانات النشر: | The American Association of Immunologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | In our efforts to improvemonoclonal antibody production, we identified novel populations of antigen-specific cells which can be classified as neither a B cell, nor an Eosinophil using classical surface markers. One novel antigen specific population is identified by the phenotype CD49b+/IgG+/IgE+/B220- (IgG/E V-cell). Immune animals have identifiable IgG/E V cell populations in multiple tissue sites including bone marrow, spleen, and blood. No IgG/E V cells were detected in the lymph nodes, thymus, or peritoneal cavity. The percentages of IgG/E V cells differ in each of the positive tissue compartments tested. Higher levels of IgG/E V cells are routinely seen in blood compared to bone marrow or spleen of immune animals. These cells have been sorted and successfully cultured on several attempts. Supernatants from sorted V cell populations were assayed for cytokine and immunoglobulin production via CBA sandwich immunoassay. No soluble IgG was detected, but low levels of IL-4 and TNF were. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::152f3be21c7b9cf58bc422e21c0f425b https://doi.org/10.4049/jimmunol.190.supp.52.21 |
| رقم الأكسشن: | edsair.doi...........152f3be21c7b9cf58bc422e21c0f425b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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