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Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transgenic Arabidopsis thaliana

التفاصيل البيبلوغرافية
العنوان: Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transgenic Arabidopsis thaliana
المؤلفون: Zhifeng Wen, Lin Wang, Bilal Ahmad, Jinhui Bai, Qingxi Chen, Muhammad Hanif, Liping Yao
المصدر: Plant Biotechnology Reports. 14:725-736
بيانات النشر: Springer Science and Business Media LLC, 2020.
سنة النشر: 2020
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Plant defensin, fungi, food and beverages, Plant Science, Genetically modified crops, Biology, Plant disease resistance, biology.organism_classification, 01 natural sciences, Microbiology, 03 medical and health sciences, 030104 developmental biology, Colletotrichum, Chitinase, biology.protein, Pseudomonas syringae, Arabidopsis thaliana, Colletotrichum higginsianum, 010606 plant biology & botany, Biotechnology
الوصف: Strawberry (Fragaria × ananassa Duch.) is one of the most widely cultivated fruit crop. Anthracnose caused by Colletotrichum spp. is a devastating disease of strawberry, causing large-scale strawberry losses worldwide. Chitinases act as defence proteins and are crucial for plant response to pathogens. Here, we isolated a class V Chitinase gene (designed as FnCHIT2, GenBank accession number MN709779) from Chinese wild diploid strawberry Fragaria nilgerrensis Schlecht (F. nilgerrensis), a species that exhibits high tolerance to anthracnose. Gene expression analysis showed that FnCHIT2 expression was highly induced after Colletotrichum gloeosporiodes inoculation and salicylic acid (SA) treatment. Subcellular localization analysis revealed the presence of FnCHIT2 in the plasma membrane. Recombinant FnCHIT2 protein was successfully expressed in E. coli Rosetta (DE3). Furthermore, we transformed FnCHIT2 into Col-0 wild type A. thaliana to perform functional analysis and evaluated the functions of Colletotrichum higginsianum and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). FnCHIT2 overexpression in A. thaliana showed enhanced resistance to C. higginsianum and Pst DC3000. Enhanced disease resistance of FnCHIT2 transgenic plants to C. higginsianum was correlated with pathogenesis-related gene 1 (AtPR1) and plant defensin 1.2 (AtPDF1.2) gene expression levels. These results provide evidence that FnCHIT2 may play an important role in response to fungal pathogens in strawberry. Our study provides an important theoretical reference for future strawberry resistance breeding.
تدمد: 1863-5474
1863-5466
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::1f22074b0b7af05050635567b21c9712
https://doi.org/10.1007/s11816-020-00648-z
حقوق: CLOSED
رقم الأكسشن: edsair.doi...........1f22074b0b7af05050635567b21c9712
قاعدة البيانات: OpenAIRE
الوصف
تدمد:18635474
18635466