A genetic platform for predicting and reducing non-tenera contamination in oil palm (Elaeis guineensis) seed supply
العنوان: | A genetic platform for predicting and reducing non-tenera contamination in oil palm (Elaeis guineensis) seed supply |
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المؤلفون: | Nathan D. Lakey, Meilina Ong-Abdullah, Rajanaidu Nookiah, Blaire Bacher, Jared M. Ordway, Peggy A. Garner, Corey Wischmeyer, Marhalil Marjuni, Melissa Beil, Eng-Ti Leslie Low, Ngoot-Chin Ting, Jerry Reed, Ravigadevi Sambanthamurthi, Mohamad Arif Abdul Manaf, Leslie Cheng-Li Ooi, Rajinder Singh, Suzana Mustaffa |
المصدر: | Tree Genetics & Genomes. 17 |
بيانات النشر: | Springer Science and Business Media LLC, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | biology, Crop yield, food and beverages, Sowing, Forestry, Horticulture, Contamination, Elaeis guineensis, biology.organism_classification, Genetics, Palm oil, Palm, Tenera, Molecular Biology, Genotyping |
الوصف: | Optimal oil palm (Elaeis guineensis) crop yields rely on the purity of the tenera fruit form. The high yielding hybrid tenera fruit form is the consequence of heterozygosity for one of nine genetic variants within the SHELL gene. High-throughput genotyping allows cost-efficient screening prior to planting to decrease unintentional non-tenera palm cultivation. We present a paradigm for dramatically reducing non-tenera cultivation by SHELL genotyping a ~ 10% sampling of seeds per seed production fruit bunch. Identification and seed supply chain removal of bunches above a predetermined non-tenera threshold represent a new paradigm for applying SHELL genetic testing in the industry. In a demonstration involving 121,896 embryos from 1304 independent dura x pisifera controlled crosses from two independent seed production units, we found that 38.4% of bunches achieved a 100% pure tenera prediction rate. The remaining bunches (61.6%) had predicted non-tenera contamination ranging from 1.0 to 89.6%, with an overall average of 3.32% seeds per bunch. SHELL genotyping of expected tenera embryos identified rare aneuploid embryos, confirmed by whole genome sequencing-based heterozygosity and copy number analyses. |
تدمد: | 1614-2950 1614-2942 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::210342c1b379f149a678b1c81c79983c https://doi.org/10.1007/s11295-021-01527-6 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi...........210342c1b379f149a678b1c81c79983c |
قاعدة البيانات: | OpenAIRE |
تدمد: | 16142950 16142942 |
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