Differential transcriptional activities of HLA-DR genes in the various haplotypes
| العنوان: | Differential transcriptional activities of HLA-DR genes in the various haplotypes |
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| المؤلفون: | P Louis, R Vincent, P Cavadore, J Clot, J F Eliaou |
| المصدر: | The Journal of Immunology. 153:5059-5067 |
| بيانات النشر: | The American Association of Immunologists, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | The regulation of HLA class II genes is of particular interest with regard to the modulation of the immune response. The polymorphism of their coding regions is directly involved in the specificity of the Ag presentation, and their level of expression affects the extent of T cell activation. Previously, we have described an allelic polymorphism in the proximal promoter regions of HLA-DRB genes. The aim of this study was to compare the transcriptional activities of the promoters of the DRB genes and DRB1 alleles in a transient expression system. We have demonstrated a marked difference in their promoter strengths, as determined by their relative abilities to initiate transcription of the chloramphenicol acetyltransferase reporter gene in human B cell lines. The polymorphism of the promoter regions has been mapped to the regulatory boxes, and, by using gel retardation experiments, we found a differential ability of the nuclear proteins to bind to the partially conserved X box regions. Taken together, our results demonstrate the functional consequences of the allelic polymorphism of the proximal promoter regions of the DRB genes. These findings strongly suggest the existence, for the HLA-DR genes, of an interdependence between the polymorphism of the coding regions, which directly affects the capacity of peptide binding, and the polymorphism of the regulatory regions, which influences the transcriptional activities of the promoters. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::332b61d5295a5ed72ce08f637a3d4d42 https://doi.org/10.4049/jimmunol.153.11.5059 |
| رقم الأكسشن: | edsair.doi...........332b61d5295a5ed72ce08f637a3d4d42 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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