التفاصيل البيبلوغرافية
العنوان:
Discrimination of the Lactobacillus acidophilus group using sequencing, species-specific PCR and SNaPshot mini-sequencing technology based on the recA gene
المؤلفون:
Fwu-Ling Lee , Lina Huang , Mu-Tzu Chang , Chien-Hsun Huang , Mu-Chiou Huang , Li-Tin Wang
المصدر:
Journal of the Science of Food and Agriculture . 92:2703-2708
بيانات النشر:
Wiley, 2012.
سنة النشر:
2012
مصطلحات موضوعية:
Genetics , Nutrition and Dietetics , Phylogenetic tree , Sequence analysis , food and beverages , Subspecies , Biology , 16S ribosomal RNA , Molecular biology , law.invention , Lactobacillus acidophilus , law , bacteria , Primer (molecular biology) , Agronomy and Crop Science , Gene , Polymerase chain reaction , Food Science , Biotechnology
الوصف:
BACKGROUND: To clearly identify specific species and subspecies of the Lactobacillus acidophilus group using phenotypic and genotypic (16S rDNA sequence analysis) techniques alone is difficult. The aim of this study was to use the recA gene for species discrimination in the L. acidophilus group, as well as to develop a species-specific primer and single nucleotide polymorphism primer based on the recA gene sequence for species and subspecies identification. RESULTS: The average sequence similarity for the recA gene among type strains was 80.0%, and most members of the L. acidophilus group could be clearly distinguished. The species-specific primer was designed according to the recA gene sequencing, which was employed for polymerase chain reaction with the template DNA of Lactobacillus strains. A single 231-bp species-specific band was found only in L. delbrueckii. A SNaPshot mini-sequencing assay using recA as a target gene was also developed. The specificity of the mini-sequencing assay was evaluated using 31 strains of L. delbrueckii species and was able to unambiguously discriminate strains belonging to the subspecies L. delbrueckii subsp. bulgaricus. CONCLUSION: The phylogenetic relationships of most strains in the L. acidophilus group can be resolved using recA gene sequencing, and a novel method to identify the species and subspecies of the L. delbrueckii and L. delbrueckii subsp. bulgaricus was developed by species-specific polymerase chain reaction combined with SNaPshot mini-sequencing. Copyright © 2012 Society of Chemical Industry
تدمد:
0022-5142
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=doi_________::3825321fb364c1acd197e728d8f0af8b https://doi.org/10.1002/jsfa.5692
حقوق:
CLOSED
رقم الأكسشن:
edsair.doi...........3825321fb364c1acd197e728d8f0af8b
قاعدة البيانات:
OpenAIRE
