Influenza-A virus specific Epstein-Barr virus lytic memory CD8 T cells in EBV sero-negative middle age adults (VIR5P.1147)
| العنوان: | Influenza-A virus specific Epstein-Barr virus lytic memory CD8 T cells in EBV sero-negative middle age adults (VIR5P.1147) |
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| المؤلفون: | Rabinarayan Mishra, Levi Watkin, Nuray Aslan, Anna Gil, Katherine Luzuriaga, Liisa Selin |
| المصدر: | The Journal of Immunology. 194:148.15-148.15 |
| بيانات النشر: | The American Association of Immunologists, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | For unknown reasons 5-10% of middle-aged adults (>35 years) remain EBV-sero-negative when the virus infects the vast majority of people, and is actively shed at high titers during chronic infection. Here we show that EBV-sero-negative (EBV-SN) HLA-A2+ middle-aged adults possess a unique IAV-M158-66(GIL) memory CD8 T cell response that cross-reacts with EBV lytic epitopes. The five tested HLA-A2+ EBV-SN middle-aged adults had a significantly increased IAV-M158-66(GIL) tetramer+ CD8 frequency compared to adult EBV sero-positive donors. Upon exposure to EBV antigens in vitro both IAV- M1(GIL) /EBV-BMLF1280-288(GLC) and IAV- M1(GIL) /EBV-BRLF1109-117(YVL), functionally cross-reactive CD8+ responses could be detected in the peripheral blood of middle-aged EBV-SN donors, while only weakly IAV-(M1)/EBV-(YVL) cross-reactive responses were detected in 4 out of 6 teenage EBV-SN donors. These IAV-M1-GIL-specific CD8 T cells in middle-aged EBV-SN adults expanded dramatically to EBV lytic antigens and produced cytokines at high functional avidity. They lysed EBV-infected targets. Additionally, these cross-reactive cells had an oligo-clonal T cell receptor repertoire different than EBV sero-positive donors. Taken together these data suggest that an altered cross-reactive T cell repertoire could mediate protective immunity against viral infection. Our results imply that sero-negative adults might have the ability to resist viral infection via heterologous immunity. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3a563314775ea3dd6f370fa42a4547e3 https://doi.org/10.4049/jimmunol.194.supp.148.15 |
| رقم الأكسشن: | edsair.doi...........3a563314775ea3dd6f370fa42a4547e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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