Novel cholinesterase inhibitory effect of α-spinasterol isolated from the leaves of Acacia auriculiformis A. CUNN Ex. Benth (Fabaceae)
| العنوان: | Novel cholinesterase inhibitory effect of α-spinasterol isolated from the leaves of Acacia auriculiformis A. CUNN Ex. Benth (Fabaceae) |
|---|---|
| المؤلفون: | Taiwo O. Elufioye, Bilqis A. Lawal, Bolatito Olanipekun, Aniefiok Udobre, A. A. Ahmadu |
| المصدر: | Tropical Journal of Pharmaceutical Research. 19:1473-1479 |
| بيانات النشر: | African Journals Online (AJOL), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Acacia auriculiformis, ABTS, Antioxidant, Traditional medicine, biology, Chemistry, DPPH, medicine.medical_treatment, Ethyl acetate, Pharmaceutical Science, Ascorbic acid, biology.organism_classification, chemistry.chemical_compound, Spinasterol, medicine, Pharmacology (medical), Trolox |
| الوصف: | Purpose: To investigate the in vitro anticholinesterase, α-glucosidase and antioxidant activities of α-spinasterol isolated from Acacia auriculiformis leaves.Methods: The powdered leaves of Acacia auriculiformis were extracted with 70 % ethanol and the dried hydroalcoholic extract was suspended in water and partitioned with ethyl acetate and n-butanol to give their soluble fractions. The in vitro inhibitory activities of α-spinasterol were determined against cholinesterase and, α-glucosidase enzymes, and free radical scavenging potentials using (1,1-diphenyl-2-picrylhydarzyl (DPPH) and 2,2-azino-bis (3-Ethylbenzothiazoline-6-sulphonic acid (ABTS) antioxidantassays.Results: The compound, α-spinasterol, exhibited moderate anticholinesterase activity (IC50 value of 44.19±2.59 μg/mL which was significantly different at (p < 0.05) when compared to the standard galanthamine (IC50 value of 1.73 ± 1.10 μg/mL). It also displayed a good α-glucosidase inhibitory activity with IC``` value of 8.65 ± 1.71μg/mL which was not significantly different when compared to the standard, acarbose with IC50 value of 2.79±0.81 μg/mL. This compound, however, exhibited weak free radical scavenging activities at 26.93 ± 0.00 and 35.16 ±.0.26 % inhibition of DPPH+ and ABTS+ radicals as compared to ascorbic acid and Trolox (73.88 ± 0.04 and 99.82 ± 0.00%) respectively.Conclusion: The results show that α-spinasterol isolated from Acacia auriculiformis exerts potent inhibitory effect against cholinesterase enzyme which might serve as a lead in the search for drugs against Alzheimer disease and diabetes mellitus. Keywords: Acacia auriculiformis, α-Spinasterol, Galanthamine, Acarbose, Trolox, Ascorbic acid |
| تدمد: | 1596-9827 1596-5996 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::5dd0dccb8f839397db8c4b2d3739c30c https://doi.org/10.4314/tjpr.v19i7.20 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........5dd0dccb8f839397db8c4b2d3739c30c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
|---|