CMTr mediated 2′-O-ribose methylation status of cap-adjacent nucleotides across animals
| العنوان: | CMTr mediated 2′-O-ribose methylation status of cap-adjacent nucleotides across animals |
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| المؤلفون: | Thomas C. Dix, Irmgard U. Haussmann, Sarah Brivio, Mohannakarthik P. Nallasivan, Yavor HadzHiev, Ferenc Müller, Berndt Müller, Jonathan Pettitt, Matthias Soller |
| المصدر: | RNA. 28:1377-1390 |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Molecular Biology |
| الوصف: | Cap methyltransferases (CMTrs) O methylate the 2′ position of the ribose (cOMe) of cap-adjacent nucleotides of animal, protist, and viral mRNAs. Animals generally have two CMTrs, whereas trypanosomes have three, and many viruses encode one in their genome. In the splice leader of mRNAs in trypanosomes, the first four nucleotides contain cOMe, but little is known about the status of cOMe in animals. Here, we show that cOMe is prominently present on the first two cap-adjacent nucleotides with species- and tissue-specific variations in Caenorhabditis elegans, honeybees, zebrafish, mouse, and human cell lines. In contrast, Drosophila contains cOMe primarily on the first cap-adjacent nucleotide. De novo RoseTTA modeling of CMTrs reveals close similarities of the overall structure and near identity for the catalytic tetrad, and for cap and cofactor binding for human, Drosophila and C. elegans CMTrs. Although viral CMTrs maintain the overall structure and catalytic tetrad, they have diverged in cap and cofactor binding. Consistent with the structural similarity, both CMTrs from Drosophila and humans methylate the first cap-adjacent nucleotide of an AGU consensus start. Because the second nucleotide is also methylated upon heat stress in Drosophila, these findings argue for regulated cOMe important for gene expression regulation. |
| تدمد: | 1469-9001 1355-8382 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::64925970f0bbb32803cb1e0bcd79b17e https://doi.org/10.1261/rna.079317.122 |
| رقم الأكسشن: | edsair.doi...........64925970f0bbb32803cb1e0bcd79b17e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14699001 13558382 |
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