Histamine skin prick tests: from established diagnostic technique to advanced experimental biomarker
| العنوان: | Histamine skin prick tests: from established diagnostic technique to advanced experimental biomarker |
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| المؤلفون: | Dorien Bamps, Katarina Berdon, Hasan Hernández, Rik Schrijvers, Jan de Hoon, KU LUVEN |
| المصدر: | Skin Pharmacology and Physiology. |
| بيانات النشر: | S. Karger AG, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Pharmacology, Physiology, Dermatology, General Medicine |
| الوصف: | Introduction Skin prick tests have a long history as diagnostic and pharmacodynamic biomarker. Besides visual assessments of the wheal and flare, objective blood flow measurements using laser Doppler imaging (LDI) and laser speckle contrast imaging (LSCI) have been reported. In light of these advancements, an up-to-date characterization of the histamine-evoked response is worthwhile. Methods A single-center study was completed in healthy males. Two parameters were addressed: (1) dermal blood flow (DBF) within a 7.65 mm ring encircling the skin prick site (DBFring), and (2) surface area of the flare (AREAflare). First, the dose response was assessed using placebo (0.9% sodium chloride) or histamine (histamine dihydrochloride 1, 3 or 10 mg/mL) skin pricks on the volar surface of subjects’ (n=12) forearm. The DBFring was measured by LDI, the AREAflare by LDI and by ruler. Secondly, the inter-arm and inter-period reproducibility of the DBFring and AREAflare, as evoked by histamine (10 mg/mL) and measured by LDI and LSCI, was examined (n=14). Lastly, the effect of aprepitant (125 mg), ketotifen (1 mg) and a single (5 mg) and fourfold (20 mg) dose of desloratadine and levocetirizine on the histamine-induced (10 mg/mL) DBFring and AREAflare was evaluated with LSCI (n=13 or 12). Results All three histamine doses induced a time-dependent vasodilation. Ruler recordings did not conclusively correlate with LDI assessments of the AREAflare. The DBFring and AREAflare were reasonably reproducible when measured by using LDI or LSCI, with negligible bias between arms and study periods and poor to moderate within-subject reproducibility (0.23≤ ICC≤ 0.71). While the fourfold dose of desloratadine (p=0.0041) and the single and fourfold dose of levocetirizine (p |
| تدمد: | 1660-5535 1660-5527 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7459f216440651275f1d734ace29c91a https://doi.org/10.1159/000528772 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........7459f216440651275f1d734ace29c91a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16605535 16605527 |
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