Cortical myosin minifilaments orchestrate the arrangement of microridge protrusions on epithelial cell surfaces
العنوان: | Cortical myosin minifilaments orchestrate the arrangement of microridge protrusions on epithelial cell surfaces |
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المؤلفون: | Aaron P. van Loon, Alvaro Sagasti, Ivan S. Erofeev, Andrew B. Goryachev |
بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | medicine.anatomical_structure, Chemistry, Apical cortex, Myosin, Zebrafish larvae, medicine, Actin, Epithelium, Cell biology |
الوصف: | Actin-based protrusions vary in morphology, stability, and arrangement on cell surfaces. Microridges are laterally-elongated protrusions arranged in maze-like patterns on mucosal epithelial cells that rearrange dynamically by fission and fusion. To characterize how microridges mature and investigate the mechanisms driving fission and fusion, we imaged microridges in the maturing skin of zebrafish larvae. After their initial development, microridges continued to lengthen and microridge alignment became increasingly well ordered. Imaging F-actin and Non-Muscle Myosin II (NMII) revealed that microridge fission and fusion were associated with local NMII activity in the apical cortex. Inhibiting NMII blocked rearrangements, reduced microridge density, and altered microridge spacing. High-resolution imaging revealed that individual cortical NMII minifilaments are tethered to protrusions, often connecting adjacent microridges. NMII minifilaments connecting the ends of microridges fused them together, whereas minifilaments oriented perpendicular to microridges severed them or pulled them closer together. Our findings demonstrate that as cells mature, microridges continue to remodel and form an increasingly orderly arrangement through a process orchestrated by cortical NMII contraction. |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7ea158be90ed2f97adf30b12dc226b8a https://doi.org/10.1101/2020.10.22.351312 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi...........7ea158be90ed2f97adf30b12dc226b8a |
قاعدة البيانات: | OpenAIRE |
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