Cells protect chromosome-microtubule attachments, independent of biorientation, using an Astrin-PP1 and CyclinB-CDK1 feedback loop
العنوان: | Cells protect chromosome-microtubule attachments, independent of biorientation, using an Astrin-PP1 and CyclinB-CDK1 feedback loop |
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المؤلفون: | Roshan L. Shrestha, Xinhong Song, Viji M. Draviam, Dominique Braun, Duccio Conti |
بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Cyclin-dependent kinase 1, Kinetochore, Microtubule, Chromosome instability, Biorientation, Chromosome, Biology, Spindle pole body, Cell biology |
الوصف: | Defects in chromosome-microtubule attachment can cause chromosomal instability, associated with infertility and aggressive cancers. Chromosome-microtubule attachment is mediated by a large macromolecular structure, the kinetochore. Kinetochore pairs are bioriented and pulled by microtubules from opposing spindle poles to ensure the equal segregation of chromosomes. Kinetochore-microtubule attachments lacking opposing-pull are detached by Aurora-B/Ipl1; yet, how mono-oriented attachments that are a prerequisite for biorientation, but lacking opposing-pull are spared is unclear. Using an RNAi-mediated screen, we uncover a unique role for the Astrin-SKAP complex in protecting mono-oriented attachments. We provide the first evidence for how a microtubule-end associated protein senses outer-kinetochore changes specific to end-on attachments and assembles into an outer kinetochore crescent to stabilise mature attachments. We find that Astrin-PP1 and Cyclin-B-CDK1 activities counteract each other to preserve mono-oriented attachments. Thus, cells are not only surveying chromosome-microtubule attachment errors, but they are also actively sensing and stabilising mature attachments independent of biorientation. |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::accb5fc8ebd386e73790cae85802ced2 https://doi.org/10.1101/2020.12.24.424312 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi...........accb5fc8ebd386e73790cae85802ced2 |
قاعدة البيانات: | OpenAIRE |
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