Septin 4 controls CCNB1 stabilization via APC/C CDC20 during meiotic G2/M transition in mouse oocytes
| العنوان: | Septin 4 controls CCNB1 stabilization via APC/C CDC20 during meiotic G2/M transition in mouse oocytes |
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| المؤلفون: | Yi Hou, Ying-Chun Ouyang, Qing-Yuan Sun, Li Chen, Jian Li, Jing-Yi Qiao, Lin Jian Gu, Zhen-Bo Wang, Heide Schatten |
| المصدر: | Journal of Cellular Physiology. 237:730-742 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Small interfering RNA, Germinal vesicle, biology, Physiology, Chemistry, Clinical Biochemistry, Maturation promoting factor, Cell Biology, CDC20, Oocyte, Septin, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Prophase, 030220 oncology & carcinogenesis, medicine, biology.protein, Microinjection |
| الوصف: | In mammals, oocytes are arrested at G2/prophase for a long time, which is called germinal vesicle (GV) arrest. After puberty, fully-grown oocytes are stimulated by a gonadotropin surge to resume meiosis as indicated by GV breakdown (GVBD). CCNB1 is accumulated to a threshold level to trigger the activation of maturation promoting factor (MPF), inducing the G2/M transition. It is generally recognized that the anaphase-promoting complex/cyclosome (APC/C) and its cofactor CDH1 (also known as FZR1) regulates the accumulation/degradation of CCNB1. Here, by using small interfering RNA (siRNA) and messenger RNA (mRNA) microinjection, immunofluorescence and confocal microscopy, immunoprecipitation, time-lapse live imaging, and immunoblotting analysis, we showed that Septin 4 regulates the G2/M transition by regulating the accumulation of CCNB1 via APC/CCDC20 . Depletion of Septin 4 caused GV arrest by reducing CCNB1 accumulation. Unexpectedly, the expression level of CDC20 was higher in Septin 4 siRNA-injected oocytes than in control oocytes, but there was no significant change in the expression level of CDH1. Importantly, the reduced GVBD after Septin 4 depletion could be rescued not only by over-expressing CCNB1 but also could be partially rescued by depleting CDC20. Taken together, our results demonstrate that Septin 4 may play a critical role in meiotic G2/M transition by indirect regulation of CCNB1 stabilization in mouse oocytes. |
| تدمد: | 1097-4652 0021-9541 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e95129595c47d3a528b1bf409241876b https://doi.org/10.1002/jcp.30498 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........e95129595c47d3a528b1bf409241876b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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