Electrospun TiO2 nanofibers are synthesized by the process of electrospinning. The Characterization by Scanning Electron Microscope (SEM) has reported the average diameter of 76.42 nm. A set of Design of Experiments (DoE) by the method of Analysis of Variance (ANOVA) is prepared which helps in determining the effect of various parameters on the nanofiber thickness. According to the one-way ANOVA results, the distance parameter showed the maximum effect on the nanofiber diameter. Interaction plots indicated, a, however, a maximum interaction between flow rate and voltage.