Hybrid Sensor Device for Simultaneous Surface Plasmon Resonance and Surface Acoustic Wave Measurements
| العنوان: | Hybrid Sensor Device for Simultaneous Surface Plasmon Resonance and Surface Acoustic Wave Measurements |
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| المؤلفون: | Electra Gizeli, Jean-Michel Friedt, Achilleas Tsortos, Anastasios G. Samarentsis, A. K. Pantazis |
| المساهمون: | Institute of Molecular Biology and Biotechnology (FORTH), Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Foundation for Research and Technology - Hellas (FORTH), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Sensors, Vol 20, Iss 6177, p 6177 (2020) Sensors Sensors, MDPI, 2020, 20 (21), pp.6177. ⟨10.3390/s20216177⟩ Volume 20 Issue 21 Sensors (Basel, Switzerland) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | [SPI.OTHER]Engineering Sciences [physics]/Other, Materials science, Love wave, Microfluidics, Phase (waves), 02 engineering and technology, lcsh:Chemical technology, PDMS microfluidics, 01 natural sciences, Biochemistry, Article, surface acoustic wave, Analytical Chemistry, lcsh:TP1-1185, Electrical and Electronic Engineering, Surface plasmon resonance, Instrumentation, business.industry, 010401 analytical chemistry, Surface acoustic wave, 3D printing, biosensors, 021001 nanoscience & nanotechnology, protein adsorption, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Amplitude, Optoelectronics, combined SPR/LW-SAW biosensing platform, 0210 nano-technology, business, Biosensor, surface plasmon resonance, Protein adsorption |
| الوصف: | Surface plasmon resonance (SPR) and Love wave (LW) surface acoustic wave (SAW) sensors have been established as reliable biosensing technologies for label-free, real-time monitoring of biomolecular interactions. This work reports the development of a combined SPR/LW-SAW platform to facilitate simultaneous optical and acoustic measurements for the investigation of biomolecules binding on a single surface. The system&rsquo s output provides recordings of two acoustic parameters, phase and amplitude of a Love wave, synchronized with SPR readings. We present the design and manufacturing of a novel experimental set-up employing, in addition to the SPR/LW-SAW device, a 3D-printed plastic holder combined with a PDMS microfluidic cell so that the platform can be used in a flow-through mode. The system was evaluated in a systematic study of the optical and acoustic responses for different surface perturbations, i.e., rigid mass loading (Au deposition), pure viscous loading (glycerol and sucrose solutions) and protein adsorption (BSA). Our results provide the theoretical and experimental basis for future application of the combined system to other biochemical and biophysical studies. |
| وصف الملف: | application/pdf |
| تدمد: | 1424-8220 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::010077acaa34578201de2ab0ce70a3a4 https://doi.org/10.3390/s20216177 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....010077acaa34578201de2ab0ce70a3a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14248220 |
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