Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats
| العنوان: | Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats |
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| المؤلفون: | Gourav Roy Choudhury, Shi Jx, Wen-Ping Lin, Jianhua Lin, Wu Jz, Wu Sq, Ke Qf, Cai B, Wu Hp, Li Wj |
| المصدر: | Spinal Cord. 54:778-784 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Green Fluorescent Proteins, Down-Regulation, Enzyme-Linked Immunosorbent Assay, Pharmacology, medicine.disease_cause, Adenoviridae, Rats, Sprague-Dawley, Small hairpin RNA, 03 medical and health sciences, 0302 clinical medicine, Bcl-2-associated X protein, Downregulation and upregulation, Animals, Medicine, Spinal cord injury, Spinal Cord Injuries, bcl-2-Associated X Protein, Neurologic Examination, biology, Tumor Necrosis Factor-alpha, business.industry, Interleukin-18, General Medicine, Flow Cytometry, medicine.disease, Rats, Disease Models, Animal, 030104 developmental biology, Neurology, Anesthesia, biology.protein, RNA Interference, Interleukin 18, Tumor necrosis factor alpha, Neurology (clinical), business, Paraplegia, Locomotion, 030217 neurology & neurosurgery |
| الوصف: | We introduced an adenoviral vector expressing interleukin-1β (IL-1β) small-hairpin RNA (shRNA) into the injured spinal cords to evaluate the therapeutic potential of IL-1β downregulation in a rat model of spinal cord injury (SCI). The purpose of this study was to investigate the possible protective effects of the IL-1β downregulation on traumatic SCI in rats. Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, People’s Republic of China. An adenoviral shRNA targeting IL-1β was constructed and injected at the T12 section 7 days before SCI. The rats’ motor functions were evaluated by the Basso–Beattie–Bresnahan (BBB) rating scale. Immunofluorescence, enzyme-linked immunosorbent assay, flow-cytometric analysis and western blots were also performed. Animals downregulating IL-1β had significantly better recovery of locomotor function and less neuronal loss after SCI. In addition, IL-1β downregulation significantly decreased tumor necrosis factor-alpha (TNF-α) level and Bax expression, reduced the activity of caspase-3 and increased Bcl-2 expression after SCI. This study demonstrated that the IL-1β downregulation may have potential therapeutic benefits for both reducing secondary damages and improving the outcomes after traumatic SCI. |
| تدمد: | 1476-5624 1362-4393 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::038ca67daf20400c7c9b8ddeeba918c5 https://doi.org/10.1038/sc.2016.20 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....038ca67daf20400c7c9b8ddeeba918c5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765624 13624393 |
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