Non‑canonical Wnt signaling contributes to ventilator‑induced lung injury through upregulation of WISP1 expression
| العنوان: | Non‑canonical Wnt signaling contributes to ventilator‑induced lung injury through upregulation of WISP1 expression |
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| المؤلفون: | Jin‑Feng Yang, Li Ming Zhang, Timothy R. Billiar, Jing Chang, Bruce R. Pitt, Yue‑Feng Xia, Wen Ouyang |
| المصدر: | International Journal of Molecular Medicine |
| بيانات النشر: | Spandidos Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Ventilator-Induced Lung Injury, Lung injury, Biology, low tidal volume, CCN Intercellular Signaling Proteins, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Proto-Oncogene Proteins, non-canonical Wnt signaling pathway, Genetics, Animals, ROCK1, Protein kinase A, Wnt Signaling Pathway, c-Jun N-terminal kinase, Regulation of gene expression, Oncogene, Kinase, Gene Expression Profiling, Wnt signaling pathway, Articles, General Medicine, respiratory system, respiratory tract diseases, WNT1-inducible secreted protein 1, 030104 developmental biology, Gene Expression Regulation, moderate tidal volume, 030220 oncology & carcinogenesis, Cancer research, Cytokines, Female, Transcriptome, Biomarkers |
| الوصف: | Mechanical ventilation may cause ventilator‑induced lung injury (VILI). Canonical Wnt signaling has been reported to serve an important role in the pathogenesis of VILI. Bioinformatics analysis revealed that canonical and non‑canonical Wnt signaling pathways were activated in VILI. However, the role of non‑canonical Wnt signaling in the pathogenesis of VILI remains unclear. The present study aimed to analyze the potential role of non‑canonical Wnt signaling in VILI pathogenesis. Lung injury was assessed via Evans blue albumin permeability and histological scoring, as well as by inflammatory cytokine expression and total protein concentration in bronchoalveolar lavage fluid. The relative protein expression of canonical and non‑canonical Wnt signaling pathway components were examined via western blotting and immunohistochemistry. The results demonstrated that 6 h of mechanical ventilation at low tidal volume (LTV; 6 ml/kg) or moderate tidal volume (MTV; 12 ml/kg) induced lung injury in sensitive A/J mice. Ventilation with MTV increased the protein levels of Wnt‑induced secreted protein 1 (WISP1), Rho‑associated protein kinase 1 (ROCK1), phosphorylated (p)‑Ras homolog gene family, member A and p‑C‑Jun N‑terminal kinase (JNK). Inhibition of ROCK1 by Y27632 and JNK by SP600125 attenuated MTV‑induced lung injury and decreased the expression of proteins involved in non‑canonical Wnt signaling, including WISP1. In conclusion, non‑canonical Wnt signaling participates in VILI by modulating WISP1 expression, which has been previously noted as critical for VILI development. Therefore, the non‑canonical Wnt signaling pathway may provide a preventive and therapeutic target in VILI. |
| تدمد: | 1791-244X 1107-3756 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03c867b95d1961454a21dc68db480626 https://doi.org/10.3892/ijmm.2019.4067 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....03c867b95d1961454a21dc68db480626 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1791244X 11073756 |
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