Association of spermatogenic failure with decreased CDC25A expression in infertile men
| العنوان: | Association of spermatogenic failure with decreased CDC25A expression in infertile men |
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| المؤلفون: | Yung Ming Lin, Chia Ling Chung, Ying Hung Lin, Ting Yi Kuo, Yu Sheng Cheng, Rui Wen Liao, Pao Lin Kuo, Yen Ni Teng, Johnny Shinn Nan Lin |
| المصدر: | Human Reproduction. 21:2346-2352 |
| بيانات النشر: | Oxford University Press (OUP), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, Spermatocyte, Biology, Testicle, Sensitivity and Specificity, Male infertility, Andrology, Testis, medicine, Humans, cdc25 Phosphatases, RNA, Messenger, Spermatogenesis, Infertility, Male, Azoospermia, DNA Primers, Microscopy, Confocal, Spermatid, Reverse Transcriptase Polymerase Chain Reaction, urogenital system, Cell Cycle, Rehabilitation, Obstetrics and Gynecology, Cell cycle, medicine.disease, Immunohistochemistry, Spermatozoa, Phenotype, medicine.anatomical_structure, Microscopy, Fluorescence, Reproductive Medicine, Sperm Retrieval |
| الوصف: | BACKGROUND: DAZ gene family is crucial for human spermatogenesis that requires the precise co-ordination of cell cycle events. CDC25A is recognized as the downstream substrate of DAZ gene family and is thought to function on the M-phase regulation of cell cycles. We investigated the expression profiles of CDC25A in the testes of infertile men and evaluated the relationship between CDC25A levels and testicular phenotype, clinical hormonal parameters and sperm retrieval results. METHODS: The protein and mRNA transcript levels of CDC25A in the testes of 40 azoospermic men were determined by immunohistochemistry and quantitative real-time-PCR. CDC25A in human spermatozoa was investigated by western blotting and immunofluorescence staining. RESULTS: The CDC25A protein was expressed mainly in spermatocyte, spermatid and spermatozoa. CDC25A transcript levels were significantly decreased (P = 0.0009) in patients with spermatogenic failure, especially in men with meiotic arrest and Sertoli cell-only syndrome. Significantly higher CDC25A transcript levels were detected in patients with successful sperm retrieval than in patients with failed sperm retrieval (P = 0.005). CONCLUSIONS: Decreased CDC25A is associated with spermatogenic failure and failed sperm retrieval in infertile men. Further studies are necessary to explore the functional roles of CDC25A in human spermatozoa. |
| تدمد: | 1460-2350 0268-1161 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05cb74796d1c2cb49ce714de33c535de https://doi.org/10.1093/humrep/del163 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....05cb74796d1c2cb49ce714de33c535de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602350 02681161 |
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