The CD8+ T Cell-Mediated Immunity Induced by HPV-E6 Uploaded in Engineered Exosomes Is Improved by ISCOMATRIXTM Adjuvant
| العنوان: | The CD8+ T Cell-Mediated Immunity Induced by HPV-E6 Uploaded in Engineered Exosomes Is Improved by ISCOMATRIXTM Adjuvant |
|---|---|
| المؤلفون: | Chiara Chiozzini, Simona Anticoli, Claudia Arenaccio, Francesco Manfredi, Adriana Baz Morelli, Maurizio Federico, Paola Di Bonito, Barbara Ridolfi |
| المساهمون: | Manfredi, Francesco, di Bonito, Paola, Ridolfi, Barbara, Anticoli, Simona, Arenaccio, Claudia, Chiozzini, Chiara, Baz Morelli, Adriana, Federico, Maurizio |
| المصدر: | Vaccines Vaccines; Volume 4; Issue 4; Pages: 42 Vaccines, Vol 4, Iss 4, p 42 (2016) |
| بيانات النشر: | MDPI, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Immunogen, medicine.medical_treatment, T cell, Immunology, lcsh:Medicine, exosomes, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, adjuvant, Drug Discovery, medicine, Cytotoxic T cell, Pharmacology (medical), Pharmacology, Nef, lcsh:R, HPV-E6, CD8+ T immune response, Microvesicles, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Adjuvant, CD8 |
| الوصف: | We recently described the induction of an efficient CD8⁺ T cell-mediated immune response against a tumor-associated antigen (TAA) uploaded in engineered exosomes used as an immunogen delivery tool. This immune response cleared tumor cells inoculated after immunization, and controlled the growth of tumors implanted before immunization. We looked for new protocols aimed at increasing the CD8⁺ T cell specific response to the antigen uploaded in engineered exosomes, assuming that an optimized CD8⁺ T cell immune response would correlate with a more effective depletion of tumor cells in the therapeutic setting. By considering HPV-E6 as a model of TAA, we found that the in vitro co-administration of engineered exosomes and ISCOMATRIX(TM) adjuvant, i.e., an adjuvant composed of purified ISCOPREP(TM) saponin, cholesterol, and phospholipids, led to a stronger antigen cross-presentation in both B- lymphoblastoid cell lines ( and monocyte-derived immature dendritic cells compared with that induced by the exosomes alone. Consistently, the co-inoculation in mice of ISCOMATRIX(TM) adjuvant and engineered exosomes induced a significant increase of TAA-specific CD8⁺ T cells compared to mice immunized with the exosomes alone. This result holds promise for effective usage of exosomes as well as alternative nanovesicles in anti-tumor therapeutic approaches. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-393X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06697b8f195426e942ead80a5cb84f9c http://europepmc.org/articles/PMC5192362 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....06697b8f195426e942ead80a5cb84f9c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2076393X |
|---|