Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression
| العنوان: | Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression |
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| المؤلفون: | Margaret A. Vizzard, Susan E. Malley, Liana Merrill |
| المصدر: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 305:R147-R156 |
| بيانات النشر: | American Physiological Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Physiology, Somatic cell, media_common.quotation_subject, Urinary Bladder, Urology, Urination, urologic and male genital diseases, Stress, Physiological, Physical Stimulation, Physiology (medical), Internal medicine, Nerve Growth Factor, Male rats, medicine, Animals, Rats, Wistar, Peroxidase, media_common, Neural Control, Urinary bladder, business.industry, Brain-Derived Neurotrophic Factor, Interstitial cystitis, medicine.disease, Urinary tract disorder, Rats, medicine.anatomical_structure, Nerve growth factor, Endocrinology, Overactive bladder, business, Stress, Psychological, Histamine |
| الوصف: | Stress exacerbates symptoms of functional lower urinary tract disorders including interstitial cystitis (IC)/bladder pain syndrome (BPS) and overactive bladder (OAB) in humans, but mechanisms contributing to symptom worsening are unknown. These studies address stress-induced changes in the structure and function of the micturition reflex using an animal model of stress in male rats. Rats were exposed to 7 days of repeated variate stress (RVS). Target organ (urinary bladder, thymus, adrenal gland) tissues were collected and weighed following RVS. Evans blue (EB) concentration and histamine, myeloperoxidase (MPO), nerve growth factor (NGF), brain-derived neurotropic factor (BDNF), and CXCL12 protein content (ELISA) were measured in the urinary bladder, and somatic sensitivity of the hindpaw and pelvic regions was determined following RVS. Bladder function was evaluated using continuous, open outlet intravesical infusion of saline in conscious rats. Increases in body weight gain were significantly ( P ≤ 0.01) attenuated by day 5 of RVS, and adrenal weight was significantly ( P ≤ 0.05) increased. Histamine, MPO, NGF, and CXCL12 protein expression was significantly ( P ≤ 0.01) increased in the urinary bladder after RVS. Somatic sensitivity of the hindpaw and pelvic regions was significantly ( P ≤ 0.01) increased at all monofilament forces tested (0.1–4 g) after RVS. Intercontraction interval, infused volume, and void volume were significantly ( P ≤ 0.01) decreased after RVS. These studies demonstrate increased voiding frequency, histamine, MPO, NGF, and CXCL12 bladder content and somatic sensitivity after RVS suggesting an inflammatory component to stress-induced changes in bladder function and somatic sensitivity. |
| تدمد: | 1522-1490 0363-6119 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06e661c055c32210bbc345252d641385 https://doi.org/10.1152/ajpregu.00089.2013 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....06e661c055c32210bbc345252d641385 |
| قاعدة البيانات: | OpenAIRE |
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