Multiresidue antibiotic-metabolite quantification method using ultra-performance liquid chromatography coupled with tandem mass spectrometry for environmental and public exposure estimation
| العنوان: | Multiresidue antibiotic-metabolite quantification method using ultra-performance liquid chromatography coupled with tandem mass spectrometry for environmental and public exposure estimation |
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| المؤلفون: | Barbara Kasprzyk-Hordern, Elizabeth Holton |
| المصدر: | Analytical and Bioanalytical Chemistry |
| بيانات النشر: | Springer Berlin Heidelberg, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Analyte, Calibration curve, Metabolite, Liquid chromatography, Wastewater-based epidemiology, Mass spectrometry, Tandem mass spectrometry, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Liquid chromatography–mass spectrometry, Limit of Detection, Tandem Mass Spectrometry, Humans, Water fingerprinting, Suspended solids, Chromatography, Selected reaction monitoring, Antibiotic, Environmental Exposure, Drug Residues, chemistry, Research Paper, Chromatography, Liquid |
| الوصف: | This manuscript describes a new multiresidue method utilising ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) via multiple reaction monitoring (MRM), for the identification and quantification of 58 antibiotics and their 26 metabolites, in various solid and liquid environmental matrices. The method was designed with a ‘one health’ approach in mind requiring multidisciplinary and multisectoral collaborative efforts. It enables comprehensive evaluation of antibiotic usage in surveyed communities via wastewater-based epidemiology, as well as allowing for the assessment of potential environmental impacts. The instrumental performance was very good, demonstrating linearity up to 3000 μg L−1, and high accuracy and precision. The method accuracy in several compounds was significantly improved by dividing calibration curves into separate ranges. This was accompanied by applying a weighting factor (1/x). Microwave-assisted and/or solid-phase extraction of analytes from liquid and solid matrices provided good recoveries for most compounds, with only a few analytes underperforming. Method quantification limits were determined as low as 0.017 ng L−1 in river water, 0.044 ng L−1 in wastewater, 0.008 ng g−1 in river sediment, and 0.009 ng g−1 in suspended solids. Overall, the method was successfully validated for the quantification of 64 analytes extracted from aqueous samples, and 45 from solids. The analytes that underperformed are considered on a semi-quantitative basis, including aminoglycosides and carbapenems. The method was applied to both solid and liquid environmental matrices, whereby several antibiotics and their metabolites were quantified. The most notable antibiotic-metabolite pairs are three sulfonamides and their N-acetyl metabolites; four macrolides/lincomycins and their N-desmethyl metabolites; and five quinolone metabolites. Graphical abstract |
| اللغة: | English |
| تدمد: | 1618-2650 1618-2642 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::071991b49d17da99cb1b46b931af6c0c http://europepmc.org/articles/PMC8425450 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....071991b49d17da99cb1b46b931af6c0c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16182650 16182642 |
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