Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity
| العنوان: | Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity |
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| المؤلفون: | Yihan Li, Jiten Manji, Daniel M. Altmann, Rosemary J. Boyton, Amy Cutler, S. Lucas Black, Catherine J. Reynolds, Zoe Webster, Deborah L. W. Chong |
| المصدر: | The Journal of Immunology Author Choice |
| بيانات النشر: | The American Association of Immunologists, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Enzyme-Linked Immunospot Assay, Regulatory T cell, T cell, Immunology, Mice, Transgenic, Biology, Transgenic Model, Interferon-gamma, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Immunity, In vivo, Novel Immunological Methods, medicine, Animals, Immunology and Allergy, Pseudomonas Infections, Transgenes, Lung, Autoimmune disease, Immunity, Cellular, medicine.disease, Acquired immune system, 3. Good health, medicine.anatomical_structure, Luminescent Measurements, Pseudomonas aeruginosa, 030215 immunology |
| الوصف: | IFN-γ is a key cytokine of innate and adaptive immunity. It is important to understand temporal changes in IFN-γ production and how these changes relate to the role of IFN-γ in diverse models of infectious and autoimmune disease, making the ability to monitor and track IFN-γ production in vivo of a substantial benefit. IFN-γ ELISPOTs have been a central methodology to measure T cell immunity for many years. In this study, we add the capacity to analyze IFN-γ responses with high sensitivity and specificity, longitudinally, in vitro and in vivo. This allows the refinement of experimental protocols because immunity can be tracked in real-time through a longitudinal approach. We have generated a novel murine IFN-γ reporter transgenic model that allows IFN-γ production to be visualized and quantified in vitro and in vivo as bioluminescence using an imaging system. At baseline, in the absence of an inflammatory stimulus, IFN-γ signal from lymphoid tissue is detectable in vivo. Reporter transgenics are used in this study to track the IFN-γ response to Pseudomonas aeruginosa infection in the lung over time in vivo. The longitudinal development of the adaptive T cell immunity following immunization with Ag is identified from day 7 in vivo. Finally, we show that we are able to use this reporter transgenic to follow the onset of autoimmune T cell activation after regulatory T cell depletion in an established model of systemic autoimmunity. This IFN-γ reporter transgenic, termed “Gammaglow,” offers a valuable new modality for tracking IFN-γ immunity, noninvasively and longitudinally over time. |
| وصف الملف: | application/pdf |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e5c9e4ad0affb7864151dd034089675 https://doi.org/10.4049/jimmunol.1801453 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0e5c9e4ad0affb7864151dd034089675 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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