G-->A hypermutation of the human immunodeficiency virus type 1 genome: evidence for dCTP pool imbalance during reverse transcription
| العنوان: | G-->A hypermutation of the human immunodeficiency virus type 1 genome: evidence for dCTP pool imbalance during reverse transcription |
|---|---|
| المؤلفون: | Monica Sala, Simon Wain-Hobson, Jean-Pierre Vartanian, Andreas Meyerhans |
| المساهمون: | Rétrovirologie Moléculaire, Institut Pasteur [Paris] (IP) |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 1994, 91 (8), pp.3092-3096. ⟨10.1073/pnas.91.8.3092⟩ |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | MESH: HIV Reverse Transcriptase, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Somatic hypermutation, MESH: Base Sequence, Biology, medicine.disease_cause, MESH: HIV-1, MESH: Base Composition, MESH: RNA-Directed DNA Polymerase, MESH: Promoter Regions, Genetic, medicine, MESH: Protein Binding, Promoter Regions, Genetic, MESH: Templates, Genetic, MESH: Mutagenesis, Genetics, Base Composition, Mutation, MESH: Molecular Sequence Data, Multidisciplinary, Base Sequence, Transition (genetics), RNA-Directed DNA Polymerase, RNA, Templates, Genetic, Nucleotidyltransferase, Molecular biology, HIV Reverse Transcriptase, Reverse transcriptase, Mutagenesis, Deoxycytosine Nucleotides, HIV-1, Primer (molecular biology), MESH: Deoxycytosine Nucleotides, Protein Binding, Research Article |
| الوصف: | The quasispecies model for RNA viruses predicts the existence of a replication error threshold beyond which there is a melting or total loss of sequence information. Retroviral G-->A hypermutation is probably an example. Here it is shown that G-->A transitions may occur in both GpG and GpA dinucleotide contexts. Transitions in GpG preferentially occur via base mispairing at the ends of runs of G residues, whereas G-->A transitions within GpA may result from temporary dislocation of the primer and template strands by a single base. The two circumstances may be related by the local dCTP substrate concentration. An in vitro elongation assay shows that primer/template dislocation is more frequent for the human immunodeficiency virus type 1 reverse transcriptase than for murine or avian retroviral enzymes. Taken together these data suggest that G-->A hypermutation is an example of induced mutation whereby the viral reverse transcriptase is forced into making errors by imbalances in the intracellular dCTP concentration. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e8d140d86dfc5f767a9ac3beafc7845 https://doi.org/10.1073/pnas.91.8.3092 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0e8d140d86dfc5f767a9ac3beafc7845 |
| قاعدة البيانات: | OpenAIRE |
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