An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody
| العنوان: | An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody |
|---|---|
| المؤلفون: | Arum Kim, Dae Gyu Kim, Sunghoon Kim, Jihye Lee, Woo Young Seo, Yoon Soo Chang, Semi Lim, Eun Joo Kang, Nam Hoon Kwon, Junsik Sung, Hyunbo Shim, Thi Thu Ha Nguyen |
| المصدر: | Biomolecules, Vol 10, Iss 820, p 820 (2020) Biomolecules Volume 10 Issue 6 Biomolecules, vol 10, iss 6 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Phage display, Lung Neoplasms, lcsh:QR1-502, Biochemistry, lcsh:Microbiology, Exon, 0302 clinical medicine, Carcinoma, Non-Small-Cell Lung, antibody, Monoclonal, Protein Isoforms, Non-Small-Cell Lung, Cells, Cultured, Cancer, Cultured, biology, medicine.diagnostic_test, Chemistry, Antibodies, Monoclonal, Nuclear Proteins, 030220 oncology & carcinogenesis, Immunohistochemistry, Rabbits, Antibody, phage display, Biotechnology, Gene isoform, medicine.drug_class, splice variant, Cells, Monoclonal antibody, Immunofluorescence, AIMP2-DX2, Antibodies, Article, Vaccine Related, 03 medical and health sciences, Cricetulus, Antigen, medicine, Animals, Humans, Molecular Biology, diagnostic marker, Prevention, Carcinoma, Molecular biology, 030104 developmental biology, biology.protein, Immunization, Biochemistry and Cell Biology |
| الوصف: | AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f3917df4ae8adbf31a69000b2aba310 https://www.mdpi.com/2218-273X/10/6/820 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0f3917df4ae8adbf31a69000b2aba310 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |