Longitudinal Analysis of Inflammatory Response to SARS-CoV-2 in the Upper Respiratory Tract Reveals an Association With Viral Load, Independent of Symptoms
| العنوان: | Longitudinal Analysis of Inflammatory Response to SARS-CoV-2 in the Upper Respiratory Tract Reveals an Association With Viral Load, Independent of Symptoms |
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| المؤلفون: | Diem-Lan Vu, Christiane S. Eberhardt, Benjamin Meyer, Claire-Anne Siegrist, Elodie von Dach, Sylvain Lemeille, Angela Huttner, Laurent Kaiser, Fiona Pigny, Arnaud M. Didierlaurent, Isabella Eckerle, Maria Vono, Paola Martinez-Murillo, Geraldine Blanchard-Rohner |
| المصدر: | Journal of clinical immunology, Vol. 41, No 8 (2021) pp. 1723-1732 Journal of Clinical Immunology |
| بيانات النشر: | Research Square Platform LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, Nasal cavity, COVID-19 / virology, medicine.medical_treatment, viruses, Immunology, Anosmia, ddc:616.07, Antibodies, Viral, Nasal wash, Virus, COVID-19 / immunology, medicine, Humans, Inflammation / etiology, Immunology and Allergy, CXCL10, Longitudinal Studies, Prospective Studies, Cytokine, Aged, Inflammation, ddc:616, Nasal Mucosa / immunology, SARS-CoV-2, business.industry, COVID-19, Middle Aged, Viral Load, Nasal Mucosa, Cytokines / biosynthesis, medicine.anatomical_structure, Symptoms, Cytokines, Original Article, Female, Nasal administration, medicine.symptom, business, Viral load, SARS-CoV-2 / immunology, Respiratory tract |
| الوصف: | Background SARS-CoV-2 infection leads to high viral loads in the upper respiratory tract that may be determinant in virus dissemination. The extent of intranasal antiviral response in relation to symptoms is unknown. Understanding how local innate responses control virus is key in the development of therapeutic approaches. Methods SARS-CoV-2-infected patients were enrolled in an observational study conducted at the Geneva University Hospitals, Switzerland, investigating virological and immunological characteristics. Nasal wash and serum specimens from a subset of patients were collected to measure viral load, IgA specific for the S1 domain of the spike protein, and a cytokine panel at different time points after infection; cytokine levels were analyzed in relation to symptoms. Results Samples from 13 SARS-CoV-2-infected patients and six controls were analyzed. We found an increase in CXCL10 and IL-6, whose levels remained elevated for up to 3 weeks after symptom onset. SARS-CoV-2 infection also induced CCL2 and GM-CSF, suggesting local recruitment and activation of myeloid cells. Local cytokine levels correlated with viral load but not with serum cytokine levels, nor with specific symptoms, including anosmia. Some patients had S1-specific IgA in the nasal cavity while almost none had IgG. Conclusion The nasal epithelium is an active site of cytokine response against SARS-CoV-2 that can last more than 2 weeks; in this mild COVID-19 cohort, anosmia was not associated with increases in any locally produced cytokines. |
| تدمد: | 0271-9142 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f8fc999b67a57d6fac36f5bf6d49ffc https://doi.org/10.21203/rs.3.rs-203414/v1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0f8fc999b67a57d6fac36f5bf6d49ffc |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 02719142 |
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