Immunomodulation mediated by a herbal syrup containing a standardized Echinacea root extract: A pilot study in healthy human subjects on cytokine gene expression
العنوان: | Immunomodulation mediated by a herbal syrup containing a standardized Echinacea root extract: A pilot study in healthy human subjects on cytokine gene expression |
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المؤلفون: | Beatrice Perissutti, Barbara Dapas, Sergio Invernizzi, Stefano Dall'Acqua, Chiara Agostinis, Dario Voinovich, Roberta Bulla, Gabriele Grassi |
المساهمون: | Dapas, Barbara, Dall'Acqua, S, Bulla, Roberta, Agostinis, Chiara, Perissutti, Beatrice, Invernizzi, S, Grassi, Gabriele, Voinovich, Dario |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Adult, Male, Echinacea Root Extract, medicine.medical_treatment, Down-Regulation, Pharmaceutical Science, Pilot Projects, Echinacea angustifolia root standardizedextract (Polinacea®)Herbal syrupImmune stimulating activityHuman gene expressionCytokinesa, Pharmacology, Plant Roots, Echinacea, Monocytes, Immunomodulation, chemistry.chemical_compound, Echinacea angustifolia root extract, Drug Discovery, medicine, Humans, Cytokine genes, Glycosides, Lymphocytes, Messenger RNA, Interleukin-6, Plant Extracts, Tumor Necrosis Factor-alpha, business.industry, Interleukin-8, Plasma levels, Middle Aged, Healthy Volunteers, Cytokine, Complementary and alternative medicine, chemistry, Immunology, Echinacoside, Cytokines, Interleukin-2, Molecular Medicine, Female, Analysis of variance, business |
الوصف: | In this study, the immunomodulatory effect of a triply standardized Echinacea angustifolia root extract (Polinacea(®)) was evaluated in 10 healthy subjects. Ten ml of syrup containing one hundred mg of extract (corresponding to 4.7 mg of Echinacoside and 8.0mg of a high molecular weight-20,000 Da- polysaccharide) were administered as a herbal syrup once a day for one month. The immunomodulatory effect was evaluated before and after herbal syrup administration evaluating the expression levels of the cytokines IL-2, IL-8, IL-6 and TNF-α. Cytokine expression was studied in lympho-monocytes and in plasma samples measuring the mRNA and protein levels, respectively. The results were analysed by ANOVA and non-parametric Friedman rank sum tests; when possible it was adopted a pair-wise comparisons at different post-treatment times, using the paired t-tests with Holm correction. The correlation between the variations of cytokine plasma levels and the respective mRNA was carried out using a linear regression model. In lympho-monocytes our data indicate the up-regulation of the mRNA levels of IL-2 and IL-8 and the down regulation of the mRNA levels of the pro-inflammatory cytokines TNF-α and IL6. The differential regulation was maximal after 14 days of treatment. IL-2 up-regulation and IL-6 down-regulation were also confirmed at the protein level in plasma. Finally, the up-regulation of the mRNA of IL-2/IL-8 and the down-regulation of IL-6 positively correlated with the protein levels detected in the plasma. In conclusion, this pilot study suggests a relevant role for the standardized Echinacea angustifolia root extract in the control of cytokine expression. This first demonstration of the immuno-modulating activity of Echinacea angustifolia root extract in the healthy subject, supports at least in part the common use of such products as health promoting supplement. |
وصف الملف: | STAMPA |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1370b0e9d0f25eae30bfd638a0e2e186 http://hdl.handle.net/11577/2996902 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....1370b0e9d0f25eae30bfd638a0e2e186 |
قاعدة البيانات: | OpenAIRE |
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