KGF regulates pulmonary epithelial proliferation and surfactant protein gene expression in adult rat lung
| العنوان: | KGF regulates pulmonary epithelial proliferation and surfactant protein gene expression in adult rat lung |
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| المؤلفون: | Robin R. Deterding, Tianli Pan, John M. Shannon, Robert J. Mason, Toshiyuki Yano, Larry D. Nielsen |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 279:L1146-L1158 |
| بيانات النشر: | American Physiological Society, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Pathology, Physiology, Thyroid Nuclear Factor 1, Gene Expression, chemistry.chemical_compound, Uteroglobin, Growth Substances, In Situ Hybridization, Pulmonary Surfactant-Associated Protein A, Age Factors, Nuclear Proteins, Hyperplasia, Pulmonary Surfactant-Associated Protein D, Specific Pathogen-Free Organisms, medicine.anatomical_structure, Keratinocyte growth factor, Pulmonary alveolus, Bronchoalveolar Lavage Fluid, Cell Division, Bromodeoxyuridine, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Fibroblast Growth Factor 7, Pulmonary Surfactant-Associated Proteins, Proteolipids, Respiratory Mucosa, In situ hybridization, Biology, Physiology (medical), medicine, Animals, Humans, RNA, Messenger, Glycoproteins, Lung, Cell growth, Proteins, Epithelial Cells, Pulmonary Surfactants, Cell Biology, medicine.disease, Molecular biology, Rats, Inbred F344, Epithelium, Rats, Fibroblast Growth Factors, Pulmonary Alveoli, chemistry, Fibroblast Growth Factor 10, Transcription Factors |
| الوصف: | Keratinocyte growth factor (KGF, FGF-7) is a potent mitogen for epithelial cells. We instilled recombinant human KGF to determine the effects of KGF on alveolar epithelial cells. Left lungs of adult rats were instilled intrabronchially with KGF (5 mg/kg) or normal saline. KGF instillation resulted in epithelial cell hyperplasia, and the alveolar bromodeoxyuridine (BrdU) labeling index peaked at 35% on day 2 after instillation. The mRNA levels for the surfactant proteins (SPs) SP-A, SP-B, and SP-D were increased in whole lung tissue on days 1 and 2 after KGF treatment and then returned to control levels on days 3–7. SP-C mRNA levels were increased on days 2–5 after KGF instillation. However, all surfactant protein mRNAs were reduced in type II cells isolated from rats instilled with KGF 2 or 3 days before isolation. These observations were confirmed by in situ hybridization. Instillation of KGF also increased the amount of SP-A and SP-D in lavage fluid. Transcripts for CC10, the 10-kDa Clara cell protein, were decreased. KGF increases the mRNA for the surfactant proteins per lung because of type II cell hyperplasia, but the mRNA per cell is slightly diminished as measured in isolated cells or estimated by in situ hybridization. |
| تدمد: | 1522-1504 1040-0605 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c4bcb177a2d1c5d81cb38b6e6a88f3 https://doi.org/10.1152/ajplung.2000.279.6.l1146 |
| رقم الأكسشن: | edsair.doi.dedup.....17c4bcb177a2d1c5d81cb38b6e6a88f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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