Multivalent cationic pseudopeptide polyplexes as a tool for cancer therapy
| العنوان: | Multivalent cationic pseudopeptide polyplexes as a tool for cancer therapy |
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| المؤلفون: | José Courty, Benoit Vallée, Ilaria Cascone, Zoi Diamantopoulou, Eric Leroy, Federica Maione, Maud-Emmanuelle Gilles, Blandine Brissault, Maha Sader, Jacques Penelle, Damien Habert, Mélissande Cossutta, Claire Houppe, Damien Destouches, Enrico Giraudo |
| المساهمون: | Croissance cellulaire, réparation et régénération tissulaires (CRRET), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et des Matériaux Paris-Est (ICMPE), Institut de Chimie du CNRS (INC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Oncotarget Oncotarget, Impact journals, 2017, 8 (52), ⟨10.18632/oncotarget.21441⟩ |
| بيانات النشر: | Impact Journals, LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Biodistribution, Pancreatic ductal adenocarcinoma, Cancer therapy, Antitumour activity, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 02 engineering and technology, 03 medical and health sciences, In vivo, Pancreatic cancer, medicine, ComputingMilieux_MISCELLANEOUS, Nucleolin, business.industry, Nanoparticles, Polyplex, Oncology, Cancer, 021001 nanoscience & nanotechnology, medicine.disease, Gemcitabine, 3. Good health, 030104 developmental biology, Immunology, Cancer research, 0210 nano-technology, business, Research Paper, medicine.drug |
| الوصف: | // Zoi Diamantopoulou 1, * , Maud-Emmanuelle Gilles 1, * , Maha Sader 1 , Melissande Cossutta 1 , Benoit Vallee 1 , Claire Houppe 1 , Damien Habert 1 , Blandine Brissault 2 , Eric Leroy 2 , Federica Maione 3 , Enrico Giraudo 3 , Damien Destouches 1 , Jacques Penelle 2 , Jose Courty 1, ** and Ilaria Cascone 1, ** 1 Laboratory of Growth, Reparation and Tissue Regeneration (CRRET), University of Paris Est, ERL-CNRS 9215, 94010 Creteil, France 2 East Paris Institute of Chemistry and Materials Science, CNRS & University Paris-Est, 94320 Thiais, France 3 Department of Oncological Sciences and Laboratory of Transgenic Mouse Models, Institute for Cancer Research and Treatment, University of Torino School of Medicine, I-10060 Candiolo, Torino, Italy * These authors have contributed equally to this work ** These authors are considered as co-senior author Correspondence to: Jose Courty, email: courty@u-pec.fr Keywords: nanoparticles, antitumour activity, polyplex, nucleolin, pancreatic ductal adenocarcinoma Received: June 21, 2017 Accepted: August 27, 2017 Published: September 30, 2017 ABSTRACT In this study, a novel anticancer reagent based on polyplexes nanoparticles was developed. These nanoparticles are obtained by mixing negatively charged polyelectrolytes with the antitumour cationically-charged pseudopeptide N6L. Using two in vivo experimental tumor pancreatic models based upon PANC-1 and mPDAC cells, we found that the antitumour activity of N6L is significantly raised via its incorporation in polyplexed nanoparticles. Study of the mechanism of action using affinity isolation and si-RNA experiments indicated that N6L-polyplexes are internalized through their interaction with nucleolin. In addition, using a very aggressive model of pancreatic cancer in which gemcitabine, a standard of care for this type of cancer, has a weak effect on tumour growth, we observed that N6L-polyplexes administration has a stronger efficacy than gemcitabine. Biodistribution studies carried out in tumour-bearing mice indicated that N6L-polyplexes localises in tumour tissue, in agreement with its antitumour effect. These results support the idea that N6L nanoparticles could develop into a promising strategy for the treatment of cancer, especially hard-to-treat pancreatic cancers. |
| تدمد: | 1949-2553 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18fe43d692118441cb915cff97634712 https://doi.org/10.18632/oncotarget.21441 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....18fe43d692118441cb915cff97634712 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19492553 |
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