Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton
| العنوان: | Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton |
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| المؤلفون: | Meng Kang, Xiaokang Fu, Liang Ma, Zhengying Lu, Jianhua Lu, Shuxun Yu, Hengling Wei, Jingjing Zhang, Hantao Wang, Xu Yang, Aimin Wu |
| المصدر: | Frontiers in Genetics, Vol 11 (2020) Frontiers in Genetics |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Na+/H+ antiporter protein, lcsh:QH426-470, Antiporter, Protein domain, Gossypium, Genome, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Genetics, Gene family, Gene, Genetics (clinical), Original Research, salt tolerance, Methyl jasmonate, biology, stress response, biology.organism_classification, lcsh:Genetics, 030104 developmental biology, chemistry, gene expansion, 030220 oncology & carcinogenesis, Molecular Medicine |
| الوصف: | The sodium/hydrogen antiporter (NHX) gene family with the Na+/H+ exchange protein domain is a transporter of sodium and hydrogen ions and plays an important role in the response of plants to salt stress. Studying the response of cotton to salt stress through comprehensive identification and analysis of NHX genes in several species and their roles in salt tolerance mechanisms is of great significance. In this study, 23, 24, 12, and 12 NHX genes were identified from Gossypium hirsutum (Gh), G. barbadense, G. arboreum and G. raimondii, respectively. Phylogenetic analysis showed that these genes were mainly divided into three clades with significant subcellular localization, namely, endosome (Endo-class), plasma membrane (PM-class) and vacuole (Vac-class). By analyzing the structure of NHX genes and proteins, each branch of the NHX gene family was found to be structurally conserved, and collinearity analysis showed that NHX genes were mainly expressed through whole genome and segmental duplication. The non-synonymous (Ka)/synonymous (Ks) values showed that the NHX gene family experienced strong purifying selection during long-term evolution. Cis-acting element analysis showed that the NHX gene family may be related to the regulation of abscisic acid (ABA) and methyl jasmonate (MeJA) hormones. Additionally, transcriptomic data analysis and qRT-PCR showed that GhNHXs exhibited different expression patterns in each tissue and under different salinities. These results provide an important reference for us to further understand and analyze the molecular regulation mechanism of cotton NHX genes. |
| اللغة: | English |
| تدمد: | 1664-8021 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b0fe7fd20a3b9fdaeafba21d13a055f https://www.frontiersin.org/article/10.3389/fgene.2020.00964/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1b0fe7fd20a3b9fdaeafba21d13a055f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16648021 |
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