التفاصيل البيبلوغرافية
العنوان:
Structures of respiratory syncytial virus nucleocapsid protein from two crystal forms: Details of potential packing interactions in the native helical form
المؤلفون:
Jingshan Ren , Kenneth L. Powell , Michael Lockyer , Nicola G. A. Abrescia , B. Dhaliwal , K. El Omari , Alastair R. Hawkins , David K. Stammers
سنة النشر:
2016
مصطلحات موضوعية:
Models, Molecular , viruses , Biophysics , Crystal structure , Biology , Crystallography, X-Ray , Biochemistry , Genome , Protein Structure, Secondary , Virus , Crystal , Protein structure , Structural Biology , Genetics , Structural Communications , Protein Interaction Domains and Motifs , Protein Structure, Quaternary , RNA , Nucleocapsid Proteins , Condensed Matter Physics , Virology , Viral replication , Respiratory Syncytial Virus, Human , Phosphoprotein , RNA, Viral
الوصف:
Respiratory syncytial virus (RSV) is a frequent cause of respiratory illness in infants, but there is currently no vaccine nor effective drug treatment against this virus. The RSV RNA genome is encapsidated and protected by a nucleocapsid protein; this RNA-nucleocapsid complex serves as a template for viral replication. Interest in the nucleocapsid protein has increased owing to its recent identification as the target site for novel anti-RSV compounds. The crystal structure of human respiratory syncytial virus nucleocapsid (HRSVN) was determined to 3.6 Å resolution from two crystal forms belonging to space groups P212121 and P1, with one and four decameric rings per asymmetric unit, respectively. In contrast to a previous structure of HRSVN, the addition of phosphoprotein was not required to obtain diffraction-quality crystals. The HRSVN structures reported here, although similar to the recently published structure, present different molecular packing which may have some biological implications. The positions of the monomers are slightly shifted in the decamer, confirming the adaptability of the ring structure. The details of the inter-ring contacts in one crystal form revealed here suggest a basis for helical packing and that the stabilization of native HRSVN is via mainly ionic interactions. © 2011 International Union of Crystallography All rights reserved.
اللغة:
English
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fdeda3070740daee24527bfe2773bdc https://doi.org/10.1107/s1744309111029228
حقوق:
OPEN
رقم الأكسشن:
edsair.doi.dedup.....1fdeda3070740daee24527bfe2773bdc
قاعدة البيانات:
OpenAIRE
