Seasonality and photoperiod influence in vitro production of buffalo embryos
| العنوان: | Seasonality and photoperiod influence in vitro production of buffalo embryos |
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| المؤلفون: | Qaisar Shahzad, Armughan Ahmed Wadood, Long Xie, Huiyan Xu, Kailin Yang, Muhammad Waqas, Y. Q. Lu, Liping Pu, Kehuan Lu, Jinguyang Wang, A.U. Husna |
| المصدر: | Reproduction in Domestic Animals. 55:1115-1123 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Buffaloes, Photoperiod, Embryonic Development, Fertilization in Vitro, Biology, 03 medical and health sciences, Polar body, 0302 clinical medicine, Endocrinology, Animal science, Seasonal breeder, medicine, Animals, HSP90 Heat-Shock Proteins, Blastocyst, photoperiodism, 030219 obstetrics & reproductive medicine, Hatching, 0402 animal and dairy science, Embryo, 04 agricultural and veterinary sciences, Oocyte, 040201 dairy & animal science, In Vitro Oocyte Maturation Techniques, In vitro maturation, medicine.anatomical_structure, Gene Expression Regulation, Oocytes, Female, Animal Science and Zoology, Seasons, Octamer Transcription Factor-3, Biotechnology |
| الوصف: | Buffalo is considered short-day breeder in tropical and subtropical part of the world and seasonality and photoperiodism impart major influence on its fertility. However, its impact on in vitro embryo production (IVEP) remains elusive. Therefore, this study investigated the effect of seasonal variations and photoperiodism on morphological and molecular parameters of IVEP in buffalo. For this purpose, we conducted two different experiments on the oocytes obtained by aspirating follicles from abattoir derived ovaries. In Exp. I, retrospective analysis was performed for oocyte recovery, blastocyst and hatching rate, during four consecutive seasonal periods (i.e. January-March, April-June, July-September and October-December). In Exp. II, oocytes from peak breeding and non-breeding seasons were subjected to 24 hr in vitro maturation and evaluated for polar body extrusion to assess maturation rate. Results showed that embryo development was markedly low during second quarter (April-June) and maximum during fourth quarter (October-December) of the year; referred as non-breeding and breeding seasons, respectively. Comparative data analysis demonstrated that poor oocyte quality is major reason for lesser efficiency of embryo production during non-breeding season than peak breeding season as suggested by poor oocyte recovery (2.31 ± 0.10 vs. 3.65 ± 0.27) and maturation rate (33.32 ± 2.1 vs. 63.15 ± 7.31). Subsequently, comparative gene expression analysis of blastocysts during peak breeding season significantly upregulated pluripotency gene (OCT-4) and downregulated heat shock protein 90, as compared to non-breeding season. Therefore, it could be divulged from the present study that seasonal variations and photoperiodism have profound effect on oocyte quality and subsequent embryo development. It is recommended to find suitable additives for in vitro maturation that could mitigate seasonal effects. |
| تدمد: | 1439-0531 0936-6768 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2077519f1f49910956e02f5b626734d6 https://doi.org/10.1111/rda.13749 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2077519f1f49910956e02f5b626734d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390531 09366768 |
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