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MicroRNA-145 engineered bone marrow-derived mesenchymal stem cells alleviated erectile dysfunction in aged rats

التفاصيل البيبلوغرافية
العنوان: MicroRNA-145 engineered bone marrow-derived mesenchymal stem cells alleviated erectile dysfunction in aged rats
المؤلفون: Tao Qi, Ke Li, Qiwei Liu, Zhansen Huang, Yubin Cui, Haojian Lin, Bo Wang, Daoyuan Hu, Jun Chen, Hengjun Xiao
المصدر: Stem Cell Research & Therapy
Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-10 (2019)
بيانات النشر: Springer Science and Business Media LLC, 2019.
سنة النشر: 2019
مصطلحات موضوعية: Male, 0301 basic medicine, MMP2, Medicine (miscellaneous), Smad2 Protein, Bone marrow-derived mesenchymal stem cells, Desmin, Rats, Sprague-Dawley, 0302 clinical medicine, Erectile Dysfunction, Medicine, lcsh:QD415-436, 3' Untranslated Regions, lcsh:R5-920, Penile Erection, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Molecular Medicine, Stem cell, lcsh:Medicine (General), Mean arterial pressure, medicine.medical_specialty, Age-associated erectile dysfunction, Kruppel-Like Transcription Factors, Urology, Down-Regulation, Mesenchymal Stem Cell Transplantation, Biochemistry, Genetics and Molecular Biology (miscellaneous), Collagen Type I, lcsh:Biochemistry, Kruppel-Like Factor 4, 03 medical and health sciences, stomatognathic system, Animals, business.industry, Research, Mesenchymal stem cell, Mesenchymal Stem Cells, Cell Biology, miR-145, medicine.disease, Actins, Rats, Transplantation, Disease Models, Animal, MicroRNAs, Collagen, type I, alpha 1, 030104 developmental biology, Erectile dysfunction, Bone marrow, business
الوصف: Background Aging is one of the dominant factors contributing to erectile dysfunction (ED), and effective treatments for age-associated ED are urgently demanded. In this study, the therapeutic efficiency of bone marrow-derived mesenchymal stem cells (BMSCs) overexpressing microRNA-145 (miR-145) was evaluated in ED. Methods Sixty male Sprague-Dawley rats (24 months old) were randomly divided into 4 treatment groups (n = 15/group): PBS (control), BMSCs, BMSCs transfected with a blank vector (vector-BMSCs), and BMSCs transfected with a lentivirus overexpressing miR-145 (OE-miR-145-BMSCs). Fourteen days after transplantation of BMSCs, erectile function was evaluated by measuring intra-cavernous pressure (ICP) and mean arterial pressure (MAP). Subsequently, penile erectile tissues were harvested and subjected to Masson staining, qRT-PCR, immunofluorescence staining, dual luciferase assay, and Western blot analysis. Results Fourteen days after transplantation, the ICP/MAP was 0.79 ± 0.05 in the OE-miR-145-BMSC group, 0.61 ± 0.06 in the BMSC group, 0.57 ± 0.06 in the vector-BMSC group, and 0.3 ± 0.01 in the PBS group. Treatment with OE-miR-145-BMSCs significantly improved ED (P P P P Conclusions MicroRNA-145 engineered BMSCs effectively attenuate age-related ED. Transplantation of miR-145-overexpressing BMSCs may provide a promising novel avenue for age-associated ED therapy.
تدمد: 1757-6512
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22f24b95dbffcf7ce1cac2e5b9aa341f
https://doi.org/10.1186/s13287-019-1509-1
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....22f24b95dbffcf7ce1cac2e5b9aa341f
قاعدة البيانات: OpenAIRE
الوصف
تدمد:17576512