Transferability and reproducibility of exposed air-liquid interface co-culture lung models
العنوان: | Transferability and reproducibility of exposed air-liquid interface co-culture lung models |
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المؤلفون: | Hedwig M. Braakhuis, Eric R. Gremmer, Anne Bannuscher, Barbara Drasler, Sandeep Keshavan, Barbara Rothen-Rutishauser, Barbara Birk, Andreas Verlohner, Robert Landsiedel, Kirsty Meldrum, Shareen H. Doak, Martin J.D. Clift, Johanna Samulin Erdem, Oda A.H. Foss, Shanbeh Zienolddiny-Narui, Tommaso Serchi, Elisa Moschini, Pamina Weber, Sabina Burla, Pramod Kumar, Otmar Schmid, Edwin Zwart, Jolanda P. Vermeulen, Rob J. Vandebriel |
سنة النشر: | 2023 |
مصطلحات موضوعية: | Inter-laboratory comparison, Toxicity, Materials Science (miscellaneous), Air exposure, Lung model, Public Health, Environmental and Occupational Health, Safety, Risk, Reliability and Quality, Nanomaterial, Safety Research, Air-liquid interface |
الوصف: | Overall, the results of the 7 participating laboratories are quite similar. After exposing Calu-3 alone and Calu-3 co-cultures with macrophages, no effects of lipopolysaccharide (LPS), quartz (DQ12) or titanium dioxide (TiO2) NM-105 particles on the cell viability and barrier integrity were detected. LPS exposure induced moderate cytokine release in the Calu-3 monoculture, albeit not statistically significant in most labs. In the co-culture models, most laboratories showed that LPS can significantly induce cytokine release (IL-6, IL-8 and TNF-α). The exposure to quartz and TiO2 particles did not induce a statistically significant increase in cytokine release in both cell models probably due to our relatively low deposited doses, which were inspired by in vivo dose levels. The intra- and inter-laboratory comparison study indicated acceptable interlaboratory variation for cell viability/toxicity (WST-1, LDH) and transepithelial electrical resistance, and relatively high inter-laboratory variation for cytokine production. |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::245bd559325ffda2512c00b83f58e046 http://hdl.handle.net/10029/626726 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....245bd559325ffda2512c00b83f58e046 |
قاعدة البيانات: | OpenAIRE |
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