One Assay for All: Exploring Small Molecule Phosphorylation Using Amylose–Polyiodide Complexes
| العنوان: | One Assay for All: Exploring Small Molecule Phosphorylation Using Amylose–Polyiodide Complexes |
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| المؤلفون: | Fang F Liu, Louis P. Conway, Huan Chen, Josef Voglmeir, Li Liu, Zhi P. Cai, Xu C. Duan, Shuang Wei |
| المصدر: | Analytical Chemistry. 87:9546-9550 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | chemistry.chemical_classification, biology, Kinase, Phosphotransferases, Substrate (chemistry), Small molecule, Analytical Chemistry, Adenosine diphosphate, chemistry.chemical_compound, Enzyme, chemistry, Biochemistry, biology.protein, Sucrose synthase, Phosphorylation, Colorimetry, Amylose, Glycogen synthase, Iodine |
| الوصف: | We present a generic method for screening small molecule kinases for their acceptor specificity. The release of the reaction byproduct adenosine diphosphate (ADP) triggers a concentration-dependent formation of amylose from sucrose, by using the combined enzymatic action of sucrose synthase and glycogen synthase. Kinase activities could be quantified photometrically after the formation of a dark-blue amylose-polyiodide complex. We demonstrate that this method can be used to profile both known and novel nucleotide- and sugar-kinases for their substrate specificity. Using a facile and widely available methodology, the amylose-polyiodide small-molecule kinase assay presented herein has the potential to perform substrate screenings of small molecule kinases in a high-throughput manner. |
| تدمد: | 1520-6882 0003-2700 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24868fca0131dc0cafa2b2a361e6a402 https://doi.org/10.1021/acs.analchem.5b02247 |
| رقم الأكسشن: | edsair.doi.dedup.....24868fca0131dc0cafa2b2a361e6a402 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15206882 00032700 |
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