MAGP-1 and fibronectin control EGFL7 functions by driving its deposition into distinct endothelial extracellular matrix locations
| العنوان: | MAGP-1 and fibronectin control EGFL7 functions by driving its deposition into distinct endothelial extracellular matrix locations |
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| المؤلفون: | Elisabeth Werkmeister, Robert P. Mecham, Nelson Dusetti, Thomas J. Broekelmann, Odile Gayet, Gaëlle Villain, Fabrice Soncin, Chantal Havet, Etienne Lelièvre, Virginie Mattot |
| المساهمون: | Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Mécanismes de tumorigenèse et thérapies ciblées, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Washington University School of Medicine [Saint Louis, MO], Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), CHU Lille, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), SONCIN, Fabrice |
| المصدر: | FEBS Journal FEBS Journal, 2018, 285 (23), pp.4394-4412. ⟨10.1111/febs.14680⟩ FEBS Journal, Wiley, 2018, 285 (23), pp.4394-4412. ⟨10.1111/febs.14680⟩ |
| بيانات النشر: | HAL CCSD, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, EGF Family of Proteins, RHOA, Leukocyte adhesion molecule, [SDV]Life Sciences [q-bio], extracellular matrix, Notch signaling pathway, Lysyl oxidase, Endothelial Growth Factors, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Biochemistry, Protein-Lysine 6-Oxidase, Extracellular matrix, 03 medical and health sciences, Contractile Proteins, fibronectin, Basic Helix-Loop-Helix Transcription Factors, Cell Adhesion, Human Umbilical Vein Endothelial Cells, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Humans, MAGP-1, Molecular Biology, Extracellular Matrix Proteins, Receptors, Notch, biology, Chemistry, Calcium-Binding Proteins, Cell Biology, Fibronectins, Cell biology, Repressor Proteins, Endothelial stem cell, Fibronectin, 030104 developmental biology, biology.protein, EGFL7, RNA Splicing Factors, Signal Transduction |
| الوصف: | International audience; The extracellular matrix (ECM) is essential to provide mechanical support to tissues but is also a bioactive edifice which controls cell behavior. Cell signaling generated by ECM components through integrin-mediated contacts, modulates cell biological activity. In addition, by sequestrating or releasing growth factors, the ECM is an active player of physiological and pathological processes such as vascular development. EGFL7 is mainly expressed during blood vessel development and is deposited in the ECM after secretion by endothelial cells. While EGFL7 is known to control various endothelial cell molecular mechanisms [i.e., the repression of endothelial-derived lysyl oxidase (LOX) enzyme, the regulation of the Notch pathway, and the expression of leukocyte adhesion molecules and of RHOA by endothelial cells], it is not established whether EGFL7 functions when bound to the ECM. Here, we show that microfibrillar-associated glycoprotein-1 (MAGP-1) and fibronectin drive the deposition of EGFL7 into both fibers and individual aggregates in endothelial ECM. Although EGFL7 does not need to be docked into the ECM to control endothelial adhesion molecule expression, the ECM accumulation of EGFL7 is required for its regulation of LOX activity and of HEY2 expression along the Notch pathway. The interaction of EGFL7 with MAGP-1 is necessary for LOX activity repression by EGFL7 while it does not participate in the control of the Notch pathway by this protein. Altogether, this study highlights the roles played by EGFL7 in controlling various endothelial molecular mechanisms upon its localization and shows how the ECM can modulate its functions. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1742-464X 1742-4658 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26df1268c128bdb6996c471b72b6f512 https://www.hal.inserm.fr/inserm-03806651/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....26df1268c128bdb6996c471b72b6f512 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1742464X 17424658 |
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