Caspase 3‐like protease is involved in ethylene‐induced programmed cell death during aerenchyma formation in Helianthus annuus stem
العنوان: | Caspase 3‐like protease is involved in ethylene‐induced programmed cell death during aerenchyma formation in Helianthus annuus stem |
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المؤلفون: | Xi‐Lu Ni, Hui Hou, Qinmi Xie, Haiying Zhang, Peixuan Yan, Yingze Lv |
المصدر: | Microscopy Research and Technique. 85:3707-3715 |
بيانات النشر: | Wiley, 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Cyclopropanes, Oxygen, Medical Laboratory Technology, Histology, Caspase 3, Helianthus, Apoptosis, Ethylenes, Anatomy, Plant Roots, Instrumentation, Peptide Hydrolases |
الوصف: | Previous research has reported that hypoxic conditions and ethylene treatments greatly trigger programmed cell death (PCD) occurrence and induce the formation of aerenchyma to adapt stress environment in Helianthus annuus stem. Caspase 3-like protease (CLP) as regulatory signals, also be involved in the process of PCD to adapt the low oxygen environment. However, the relationships between ethylene and CLP have seldom been reported. Herein, To understand the regulatory role of ethylene and CLP signaling molecules in aerenchyma formation, we investigated the effects of exogenous ethephon (ET), ethylene perception inhibitor 1-methylcyclopropene (1-MCP), and the treatment of 1-MCP + ET on morphological, physiological characteristics and aerenchyma formation in H. annuus stem. The results showed that lysigenous aerenchyma formation in H. annuus stem is induced by ET, and immunohistochemistry assay indicate CLP activity is raised at the formation stage of aerenchyma formation, and decreased at the expanding phase of aerenchyma formation. Western blotting illustrate the expression of CLP is also increased within 8 h after ethylene signaling inducing aerenchyma formation, and the activities of CLP are higher in ET treated seedlings than the control and 1-MCP treated seedlings. The same phenomenon was also observed by caspase-3 activity assay. These results revealed there is a causal and interdependent relationship between ET and CLP signaling during the process of aerenchyma formation, which regulating PCD initiation in H. annuus stem. |
تدمد: | 1097-0029 1059-910X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bc4e15a6741186a6ef9d696b55e4ded https://doi.org/10.1002/jemt.24222 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....2bc4e15a6741186a6ef9d696b55e4ded |
قاعدة البيانات: | OpenAIRE |
تدمد: | 10970029 1059910X |
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