A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection
| العنوان: | A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection |
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| المؤلفون: | Kazunori Tanaka, Shinji Hadano, Kaori Yasutake, Joh-E Ikeda, Takuya Kanno |
| المصدر: | PLoS ONE, Vol 12, Iss 10, p e0186227 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Central Nervous System, alpha-2-HS-Glycoprotein, Glycobiology, lcsh:Medicine, Pharmacology, Biochemistry, Nervous System, Motor Neuron Diseases, Mice, Hemopexin, Acetamides, Medicine and Health Sciences, N-Linked Glycoproteins, lcsh:Science, chemistry.chemical_classification, Neurons, Neuronal Death, Multidisciplinary, biology, Cell Death, Pharmaceutics, Imidazoles, Cell Differentiation, Neurodegenerative Diseases, Separation Processes, Neuroprotective Agents, Neurology, Cell Processes, Anatomy, Research Article, Programmed cell death, Glycan, Research and Analysis Methods, Neuroprotection, Small Molecule Libraries, 03 medical and health sciences, Animals, Humans, Glycoproteins, 030102 biochemistry & molecular biology, Amyotrophic Lateral Sclerosis, lcsh:R, Biology and Life Sciences, Cell Biology, Elution, In vitro, Culture Media, Oxidative Stress, 030104 developmental biology, chemistry, biology.protein, Cattle, lcsh:Q, Glycoprotein, Drug Delivery, alpha-2-HS-glycoprotein, Fetal bovine serum, Developmental Biology |
| الوصف: | Therapeutic agents to the central nervous system (CNS) need to be efficiently delivered to the target site of action at appropriate therapeutic levels. However, a limited number of effective drugs for the treatment of neurological diseases has been developed thus far. Further, the pharmacological mechanisms by which such therapeutic agents can protect neurons from cell death have not been fully understood. We have previously reported the novel small-molecule compound, 2-[mesityl(methyl)amino]-N-[4-(pyridin-2-yl)-1H-imidazol-2-yl] acetamide trihydrochloride (WN1316), as a unique neuroprotectant against oxidative injury and a highly promising remedy for the treatment of amyotrophic lateral sclerosis (ALS). One of the remarkable characteristics of WN1316 is that its efficacious doses in ALS mouse models are much less than those against oxidative injury in cultured human neuronal cells. It is also noted that the WN1316 cytoprotective activity observed in cultured cells is totally dependent upon the addition of fetal bovine serum in culture medium. These findings led us to postulate some serum factors being tightly linked to the WN1316 efficacy. In this study, we sieved through fetal bovine serum proteins and identified two N-linked glycoproteins, alpha-2-HS-glycoprotein (AHSG) and hemopexin (HPX), requisites to exert the WN1316 cytoprotective activity against oxidative injury in neuronal cells in vitro. Notably, the removal of glycan chains from these molecules did not affect the WN1316 cytoprotective activity. Thus, two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::302ddf160e3982b84de009c18d0b8cbc http://europepmc.org/articles/PMC5633190?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....302ddf160e3982b84de009c18d0b8cbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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