Unusual Features of Vaccinia Virus Extracellular Virion Form Neutralization Resistance Revealed in Human Antibody Responses to the Smallpox Vaccine
| العنوان: | Unusual Features of Vaccinia Virus Extracellular Virion Form Neutralization Resistance Revealed in Human Antibody Responses to the Smallpox Vaccine |
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| المؤلفون: | Dirk M. Zajonc, Steven R. Head, Dennis R. Burton, Philip L. Felgner, Bjoern Peters, Matthew Maybeno, Rowena O. Aguilar-Sino, Yan Xiang, Lilia Koriazova, James E. Crowe, David Blum, Mohammed Rafii El Idrissi Benhnia, Michael H. Matho, Xiangzhi Meng, Shin-ichiro Kato, Shane Crotty |
| المصدر: | Journal of Virology. 87:1569-1585 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.drug_class, Viral pathogenesis, Immunology, Vaccinia virus, Biology, Antibodies, Viral, Monoclonal antibody, Microbiology, Virus, Neutralization, chemistry.chemical_compound, Neutralization Tests, Virology, medicine, Humans, Smallpox vaccine, Antigens, Viral, Virion, Antibodies, Monoclonal, Complement System Proteins, Antibodies, Neutralizing, chemistry, Viral Receptor, Insect Science, biology.protein, Pathogenesis and Immunity, Vaccinia, Antibody, Smallpox Vaccine |
| الوصف: | The extracellular virion form (EV) of vaccinia virus (VACV) is essential for viral pathogenesis and is difficult to neutralize with antibodies. Why this is the case and how the smallpox vaccine overcomes this challenge remain incompletely understood. We previously showed that high concentrations of anti-B5 antibodies are insufficient to directly neutralize EV (M. R. Benhnia, et al., J. Virol. 83:1201–1215, 2009). This allowed for at least two possible interpretations: covering the EV surface is insufficient for neutralization, or there are insufficient copies of B5 to allow anti-B5 IgG to cover the whole surface of EV and another viral receptor protein remains active. We endeavored to test these possibilities, focusing on the antibody responses elicited by immunization against smallpox. We tested whether human monoclonal antibodies (MAbs) against the three major EV antigens, B5, A33, and A56, could individually or together neutralize EV. While anti-B5 or anti-A33 (but not anti-A56) MAbs of appropriate isotypes were capable of neutralizing EV in the presence of complement, a mixture of anti-B5, anti-A33, and anti-A56 MAbs was incapable of directly neutralizing EV, even at high concentrations. This remained true when neutralizing the IHD-J strain, which lacks a functional version of the fourth and final known EV surface protein, A34. These immunological data are consistent with the possibility that viral proteins may not be the active component of the EV surface for target cell binding and infectivity. We conclude that the protection afforded by the smallpox vaccine anti-EV response is predominantly mediated not by direct neutralization but by isotype-dependent effector functions, such as complement recruitment for antibodies targeting B5 and A33. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c1cc7dd619d4c72ef7c3c37c0f076d https://doi.org/10.1128/jvi.02152-12 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....30c1cc7dd619d4c72ef7c3c37c0f076d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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