A standard tissue as a control for histochemical and immunohistochemical staining
| العنوان: | A standard tissue as a control for histochemical and immunohistochemical staining |
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| المؤلفون: | Jerry Fredenburgh, Dennis Otali, William E. Grizzle, Denise K. Oelschlager |
| المصدر: | Biotechnic & Histochemistry. 91:309-326 |
| بيانات النشر: | Informa UK Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Histology, H&E stain, Periodic acid–Schiff stain, Biology, Carmine, Stain, Article, Masson's trichrome stain, 03 medical and health sciences, 0302 clinical medicine, Neoplasms, medicine, Humans, Staining and Labeling, 030102 biochemistry & molecular biology, General Medicine, Cadherins, Immunohistochemistry, Staining, Medical Laboratory Technology, Specimen collection, 030220 oncology & carcinogenesis, Keratins, Biomarkers, Immunostaining |
| الوصف: | The variable quality of histochemical and immunohistochemical staining of tissues may be attributed to pre-analytical and analytical variables. Both categories of variables frequently are undefined or inadequately controlled during specimen collection and preparation. Pre-analytical variables may alter the molecular composition of tissues, which results in variable staining; such variations may cause problems when different tissues are used as staining controls. We developed a standard tissue for use as a staining control. Our standard tissue contains five components: 1) nine combined human cell lines mixed with stroma from human spleen; 2) a squamous cancer cell line, A431; 3) fungus; 4) transverse sections of the mosquitofish and 5) normal human spleen. The first three components were embedded in HistoGel(™) and all components were processed to paraffin and used to construct a single standard paraffin block. The muscles of mosquitofish and arteries of the spleen are positive controls for eosin staining, while other tissues are useful for assessing hematoxylin staining. The mosquitofish tissues also are excellent controls for the Masson trichrome stain and all mucin-related histochemical stains that we tested. The goblet cells of the intestine and skin stained strongly with Alcian blue, pH 2.5 (AB-2.5), mucicarmine, colloidal iron, periodic acid Schiff (PAS) or PAS-hematoxylin (PASH) and combination stains such as colloidal iron-PASH. Cell lines were not useful for evaluating histochemical stains except for PASH. The splenic stroma was a useful control for AB-2.5; however, eosin and mucin stains stained cell lines poorly, probably due to their rapid growth and associated loss of some differentiated characteristics such as production of mucins. Nevertheless, the cell lines were a critical control for immunohistochemical stains. Immunostaining of specific cell lines was consistent with the presence of markers, e.g., EGFr in DU145 cells. The cell lines expressed a wide range of markers, so they were useful controls for immunohistochemical staining including EGFr, HER2, E-cadherin, cytokeratins, Ki67, PCNA, estrogen receptor, progesterone receptor, CD3, CD20 and CD45, activated (cleaved) caspase 3 and Bcl-2. The cell lines also were a control for the TUNEL stain. |
| تدمد: | 1473-7760 1052-0295 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3129ba9a025ca3e55b2bb3182eadd8d4 https://doi.org/10.1080/10520295.2016.1179342 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3129ba9a025ca3e55b2bb3182eadd8d4 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 14737760 10520295 |
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