Binding of von Willebrand Factor to Collagen by Flow Cytometry
| العنوان: | Binding of von Willebrand Factor to Collagen by Flow Cytometry |
|---|---|
| المؤلفون: | Gonzalo A. Carballo, Adriana I. Woods, Cristina Elena Farias, Maria A. Lazzari, María R. Silaf, Ana Catalina Kempfer |
| المصدر: | American Journal of Clinical Pathology. 111:418-423 |
| بيانات النشر: | Oxford University Press (OUP), 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | congenital, hereditary, and neonatal diseases and abnormalities, Pathology, medicine.medical_specialty, Enzyme-Linked Immunosorbent Assay, Immunoelectrophoresis, Hemophilia A, Sensitivity and Specificity, Flow cytometry, Von Willebrand factor, Antigen, Reference Values, hemic and lymphatic diseases, von Willebrand Factor, medicine, Von Willebrand disease, Coagulopathy, Animals, Humans, Deamino Arginine Vasopressin, Detection limit, medicine.diagnostic_test, biology, Liaison, Chemistry, General Medicine, Flow Cytometry, medicine.disease, Molecular biology, Microspheres, Molecular Weight, von Willebrand Diseases, cardiovascular system, biology.protein, Polystyrenes, Collagen, Rabbits, Protein Binding, circulatory and respiratory physiology |
| الوصف: | We developed a new method for the detection of large von Willebrand factor (vWf) multimers binding to collagen and for the determination of vWf antigen (vWf:Ag) using flow cytometry. Collagen is coated on to polystyrene beads, allowing detection of found large vWf multimers. In addition, rabbit antibody against vWf is coated on to the beads allowing detection of all vWf:Ag. In plasma samples from healthy persons and patients (with type 1, 2A, 2N, or severe von Willebrand disease or hemophilia), 4 different assays were performed: vWf:Ag by immunoelectrophoresis; vWf ristocetin cofactor (vWf:RCof); CBA; and vWf:Ag based on an enzyme-linked immunosorbent assay using polystyrene beads. We assayed the flow cytometric method using 2 bead sizes. The optimal bead size was 3.136 microns. The results of CBA and vWf:Ag closely correlated with those of vWf:RCof and vWf:Ag (immunoelectrophoresis), respectively, and showed a low limit of detection. Interassay variance of cytometric methods was lower than interassay variance of traditional assays. In addition, we used the new assays to monitor desmopressin therapy. |
| تدمد: | 1943-7722 0002-9173 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::381a3be7cf18e0bad9c7c6f23ae70300 https://doi.org/10.1093/ajcp/111.3.418 |
| رقم الأكسشن: | edsair.doi.dedup.....381a3be7cf18e0bad9c7c6f23ae70300 |
| قاعدة البيانات: | OpenAIRE |
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