c-Myc Overexpression Causes Anaplasia in Medulloblastoma
| العنوان: | c-Myc Overexpression Causes Anaplasia in Medulloblastoma |
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| المؤلفون: | Peter C. Burger, Aneeka Chaudhry, Chi V. Dang, Ty W. Abel, Duncan Stearns, Charles G. Eberhart |
| المصدر: | Cancer Research. 66:673-681 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Cancer Research, Transplantation, Heterologous, Apoptosis, Biology, Proto-Oncogene Proteins c-myc, Anaplastic Medulloblastoma, Mice, Necrosis, MYC Gene Amplification, Tumor Cells, Cultured, medicine, Animals, Humans, Cerebellar Neoplasms, Anaplasia, Cell Proliferation, Medulloblastoma, Cell growth, Large cell, Recurrent Medulloblastoma, medicine.disease, Transplantation, Oncology, Cancer research, medicine.symptom |
| الوصف: | Both anaplasia and increased c-myc gene expression have been shown to be negative prognostic indicators for survival in medulloblastoma patients. myc gene amplification has been identified in many large cell/anaplastic medulloblastoma, but no causative link between c-myc and anaplastic changes has been established. To address this, we stably overexpressed c-myc in two medulloblastoma cell lines, DAOY and UW228, and examined the changes in growth characteristics. When analyzed in vitro, cell lines with increased levels of c-myc had higher rates of growth and apoptosis as well as significantly improved ability to form colonies in soft agar compared with control. When injected s.c. into nu/nu mice, flank xenograft tumors with high levels of c-myc in DAOY cell line background were 75% larger than those derived from control. Overexpression of c-myc was required for tumor formation by UW228 cells. Most remarkably, the histopathology of the Myc tumors was severely anaplastic, with large areas of necrosis/apoptosis, increased nuclear size, and macronucleoli. Indices of proliferation and apoptosis were also significantly higher in Myc xenografts. Thus, c-myc seems to play a causal role in inducing anaplasia in medulloblastoma. Because anaplastic changes are often observed in recurrent medulloblastoma, we propose that c-myc dysregulation is involved in the progression of these malignant embryonal neoplasms. (Cancer Res 2006; 66(2): 673-81) |
| تدمد: | 1538-7445 0008-5472 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aa01191292aea177300fccd028e2528 https://doi.org/10.1158/0008-5472.can-05-1580 |
| رقم الأكسشن: | edsair.doi.dedup.....3aa01191292aea177300fccd028e2528 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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