Heterogeneity of postsynaptic receptor occupancy fluctuations among glycinergic inhibitory synapses in the zebrafish hindbrain
| العنوان: | Heterogeneity of postsynaptic receptor occupancy fluctuations among glycinergic inhibitory synapses in the zebrafish hindbrain |
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| المؤلفون: | Pascal Legendre, Carmen Ionela Badiu, Jean-Michel Rigo |
| المساهمون: | Center for Cellular and Molecular Neurobiology, Université de Liège-CNCM-Sart-Tilman, Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), This work was supported by INSERM, CNRS (P. Legendre and C. I. Badiu) and INSERM-CFB agreement (P. Legendre and J. M. Rigo). C. I. Badiu was supported by a Ministère de la Recherche fellowship. J. M. Rigo was supported by a CNRS fellowship. |
| المصدر: | The Journal of Physiology The Journal of Physiology, Wiley, 2003, 553, pp.819-832. ⟨10.1113/jphysiol.2003.049577⟩ The Journal of Physiology, 2003, 553, pp.819-832. ⟨10.1113/jphysiol.2003.049577⟩ |
| بيانات النشر: | HAL CCSD, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Patch-Clamp Techniques, Physiology, Postsynaptic Current, Spider Venoms, postsynaptic currents, Hindbrain, In Vitro Techniques, Biology, Inhibitory postsynaptic potential, 03 medical and health sciences, Receptors, Glycine, 0302 clinical medicine, Neurotransmitter receptor, Postsynaptic potential, Animals, Patch clamp, Evoked Potentials, Glycine receptor, Zebrafish, 030304 developmental biology, Neurons, 0303 health sciences, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Original Articles, Electric Stimulation, Electrophysiology, Rhombencephalon, Kinetics, postsynaptic glycine receptors, Synapses, zebrafish larvae, Neuroscience, 030217 neurology & neurosurgery |
| الوصف: | The amplitude of glycinergic miniature inhibitory postsynaptic currents (mIPSCs) varies considerably in neurons recorded in the isolated hindbrain of 50-h-old zebrafish larvae. At this age, glycinergic synapses are functionally mature. In order to measure the occupancy level of postsynaptic glycine receptors (GlyRs) and to determine the pre- and/or postsynaptic origin of its variability, we analysed mIPSCs within bursts evoked by alpha-latrotoxin (0.1-1 nM). Two types of burst were observed according to their mIPSC frequencies: 'slow' bursts with clearly spaced mIPSCs and 'fast' bursts characterised by superimposed events. Non-stationary noise analysis of mIPSCs in some 'slow' bursts recorded in the presence or in the absence of Ca2+ denoted that mIPSC amplitude variance did not depend on the quantity of neurotransmitters released (presynaptic origin), but rather on intrinsic stochastic behaviour of the same group of GlyRs (postsynaptic origin). In these bursts, the open probability measured at the peak of the mIPSCs was close to 0.5 while the maximum open probability is close to 0.9 for the synaptic isoform of GlyRs (heteromeric alpha1/beta GlyRs). In 'fast' bursts with superimposed events, a correlation was found between the amplitude of mIPSCs and the basal current level measured at their onset, which could suggest that the same group of GlyRs is activated during such bursts. Altogether, our results indicate that glycine synapses can display different release modes in the presence of alpha-latrotoxin. They also indicate that, in our model, postsynaptic GlyRs cannot be saturated by the release of a single vesicle. |
| اللغة: | English |
| تدمد: | 0022-3751 1469-7793 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3afbf79b06a2ca060c1bf18d13c28e70 https://hal.archives-ouvertes.fr/hal-00080136 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3afbf79b06a2ca060c1bf18d13c28e70 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00223751 14697793 |
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