The effects of 50 Hz magnetic field–exposed cell culture medium on cellular functions in FL cells
| العنوان: | The effects of 50 Hz magnetic field–exposed cell culture medium on cellular functions in FL cells |
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| المؤلفون: | Guangdi Chen, Yiqin Wang, Chuning Zhao, Haifeng Lou, Liling Su, Yue Fei |
| المصدر: | Journal of Radiation Research |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | culture medium, Cell Survival, MAP Kinase Signaling System, Health, Toxicology and Mutagenesis, relative permittivity, medicine.disease_cause, 50 Hz MF, Flow cytometry, Histones, 03 medical and health sciences, 0302 clinical medicine, Regular Paper, medicine, Humans, Radiology, Nuclear Medicine and imaging, Amnion, Viability assay, Protein kinase A, Biology, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Radiation, medicine.diagnostic_test, biological effects, Chemistry, Dose-Response Relationship, Radiation, Epithelial Cells, Surface Plasmon Resonance, Culture Media, Comet assay, Oxidative Stress, Magnetic Fields, Cell culture, 030220 oncology & carcinogenesis, Amniotic epithelial cells, Biophysics, Comet Assay, Signal transduction, Reactive Oxygen Species, Oxidative stress, Signal Transduction |
| الوصف: | Although extremely low frequency magnetic fields (ELF-MFs) have been classified as a possible carcinogen for humans by the International Agency for Research on Cancer (IARC), their biological effects and underlying mechanisms are still unclear. Our previous study indicated that ELF-MF exposure influenced the relative permittivity of the saline solution, suggesting that the MF exposure altered physical properties of the solution. To explore the biophysical mechanism of ELF-MF–induced biological effects, this study examined the effects of 50 Hz sinusoidal MF at 0–4.0 mT on the permittivity of culture medium with phase-interrogation surface plasmon resonance (SPR) sensing. Then, the biological effects of MF pre-exposed culture medium on cell viability, the mitogen-activated protein kinase (MAPK) signaling pathways, oxidative stress, and genetic stabilities were analyzed using Cell Counting Kit-8, western blot, flow cytometry, γH2AX foci formation, and comet assay. The results showed that SPR signals were decreased under MF exposure in a time- and dose-dependent manner, and the decreased SPR signals were reversible when the exposure was drawn off. However, MF pre-exposed culture medium did not significantly change cell viability, intracellular reactive oxygen species level, activation of the MARK signaling pathways, or genetic stabilities in human amniotic epithelial cells (FL cells). In conclusion, our data suggest that the relative permittivity of culture medium was influenced by 50 Hz MF exposure, but this change did not affect the biological processes in FL cells. |
| تدمد: | 1349-9157 0449-3060 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cf9dbfe68e17ef4e228352ad74f0ade https://doi.org/10.1093/jrr/rrz020 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3cf9dbfe68e17ef4e228352ad74f0ade |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13499157 04493060 |
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