Development of Human Vectored Brucellosis Vaccine Formulation: Assessment of Safety and Protectiveness of Influenza Viral Vectors Expressing Brucella Immunodominant Proteins in Mice and Guinea Pigs
| العنوان: | Development of Human Vectored Brucellosis Vaccine Formulation: Assessment of Safety and Protectiveness of Influenza Viral Vectors Expressing Brucella Immunodominant Proteins in Mice and Guinea Pigs |
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| المؤلفون: | Dina Bugybayeva, Nurika Assanzhanova, Makhpal K. Sarmykova, Kaissar Tabynov, Nadezhda Zinina, Zhailaubay Kydyrbayev, Sholpan Ryskeldinova |
| المصدر: | BioMed Research International, Vol 2020 (2020) BioMed Research International |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Article Subject, Drug Compounding, Genetic Vectors, Guinea Pigs, 030106 microbiology, Immunization, Secondary, Brucella Vaccine, Virulence, Brucella, medicine.disease_cause, Brucellosis, General Biochemistry, Genetics and Molecular Biology, Viral vector, law.invention, Mice, 03 medical and health sciences, law, Chlorocebus aethiops, Influenza, Human, Brucella melitensis, medicine, Animals, Humans, Vero Cells, Brucellosis vaccine, General Immunology and Microbiology, biology, Immunodominant Epitopes, Body Weight, Proteins, General Medicine, medicine.disease, biology.organism_classification, Virology, Influenza A virus subtype H5N1, 030104 developmental biology, Recombinant DNA, Medicine, Immunization, Bacterial outer membrane, Research Article |
| الوصف: | In this paper, we first used recombinant influenza viral vector (rIVV) subtype H5N1 expressing from the open reading frame of NS1 80 and NS1 124 amino acids of Brucella outer membrane proteins (Omp) 16 and 19, ribosomal L7/L12, and Cu-Zn superoxide dismutase (SOD) proteins to develop a human brucellosis vaccine. We made 18 combinations of IVVs in mono-, bi-, and tetravalent vaccine formulations and tested them on mice to select the safest and most effective vaccine samples. Then, the most effective vaccine candidates were further tested on guinea pigs. Safety of the rIVV-based vaccine candidate was evaluated by a mouse weight-gain test. Mice and guinea pigs were challenged with the virulent strain B. melitensis 16M. The protective effect of the rIVV-based vaccine candidate was assessed by quantitation of Brucella colonization in tissues and organs of challenged animals. All vaccine formulations were safe in mice. Tested vaccine formulations, as well as the commercial B. melitensis Rev.1 vaccine, have been found to protect mice from B. melitensis 16M infection within the range of 1.6 to 2.97 log10 units ( P < 0.05 ). Tetravalent vaccine formulations from the position of NS1 80 amino acids ( 0.2 ± 0.4 ), as well as the commercial B. melitensis Rev.1 vaccine ( 1.2 ± 2.6 ), have been found to protect guinea pigs from B. melitensis 16M infection at a significant level ( P < 0.05 ). Thus, tetravalent vaccine formulation Flu-NS1-80-Omp16+Flu-NS1-80-L7/L12+Flu-NS1-80-Omp19+Flu-NS1-80-SOD was chosen as a potential vaccine candidate for further development of an effective human vaccine against brucellosis. These results show a promising future for the development of a safe human vaccine against brucellosis based on rIVVs. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d9d80ec1885230f16fc63cc9f8a4b84 https://doaj.org/article/25ea10c7d7c749319873c64ac07fd2ef |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3d9d80ec1885230f16fc63cc9f8a4b84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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