Pulsatilla Saponin D Inhibits Autophagic Flux and Synergistically Enhances the Anticancer Activity of Chemotherapeutic Agents Against HeLa Cells
| العنوان: | Pulsatilla Saponin D Inhibits Autophagic Flux and Synergistically Enhances the Anticancer Activity of Chemotherapeutic Agents Against HeLa Cells |
|---|---|
| المؤلفون: | Xuejing Jia, Huanxing Su, Chao Zhang, Meiwan Chen, Jian-Bo Wan, Jiaolin Bao, Borong Huang, Chengwei He, Kai Wang, Yitao Wang, Yulin Zhang |
| المصدر: | The American Journal of Chinese Medicine. 43:1657-1670 |
| بيانات النشر: | World Scientific Pub Co Pte Lt, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, MAPK/ERK pathway, Saponin, Uterine Cervical Neoplasms, Biology, Pharmacology, HeLa, 03 medical and health sciences, 0302 clinical medicine, Autophagy, Humans, Inducer, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, PI3K/AKT/mTOR pathway, chemistry.chemical_classification, TOR Serine-Threonine Kinases, RNA-Binding Proteins, Ribosomal Protein S6 Kinases, 70-kDa, General Medicine, Saponins, biology.organism_classification, Antineoplastic Agents, Phytogenic, 030104 developmental biology, Complementary and alternative medicine, Biochemistry, chemistry, 030220 oncology & carcinogenesis, Female, Pulsatilla, Microtubule-Associated Proteins, Flux (metabolism), HeLa Cells, Phytotherapy |
| الوصف: | Pulsatilla saponin D (SB365), a saponin isolated from rhizoma of Pulsatilla chinensis (Bunge) Regel, exhibited anticancer activities in various cancer types. In the present study, we identified that SB365 was a potent inhibitor of autophagic flux in several cancer cell lines. SB365 induced a robust accumulation of autophagosomes as evidenced by monodansylaervarine (MDC) staining and increased protein levels of LC3-II. However, SB365 caused the accumulation of p62, a substrate that should be degraded through the autophagy–lysosomal pathway. These results indicated that SB365 was an inducer of autophagosome formation, but an inhibitor of autophagic flux. Interestingly, we found that SB365 synergistically enhanced the anticancer activity of chemotherapeutic agents against cervical cancer HeLa cells. Furthermore, our study demonstrated that SB365 increased the phosphorylation of ERK and inhibited the phosphorylation of mTOR and p70S6K, suggesting that their roles in the effects of SB365 on autophagy. These results suggest that SB365 could be a promising adjuvant anticancer agent. |
| تدمد: | 1793-6853 0192-415X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e394c1bb0241485714675a332319fab https://doi.org/10.1142/s0192415x15500949 |
| رقم الأكسشن: | edsair.doi.dedup.....3e394c1bb0241485714675a332319fab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17936853 0192415X |
|---|