Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells
| العنوان: | Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells |
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| المؤلفون: | Kimberly Dine, Ashley Ellis, Jodie L. Sauer, Reas S. Khan, Larry R. Brown, Ziv Z. Kirshner, Howard Wessel, Keirnan Willett, Kenneth S. Shindler |
| المصدر: | PLoS ONE, Vol 16, Iss 1, p e0243862 (2021) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Retinal Ganglion Cells, Macroglial Cells, 0302 clinical medicine, Animal Cells, Medicine and Health Sciences, Immune Response, Neurons, Multidisciplinary, Chemistry, Experimental autoimmune encephalomyelitis, Animal Models, Neuroprotection, Cell biology, medicine.anatomical_structure, Retinal ganglion cell, Experimental Organism Systems, Optic nerve, Medicine, Female, medicine.symptom, Cellular Types, Anatomy, Research Article, Ganglion Cells, Encephalomyelitis, Autoimmune, Experimental, Optic Neuritis, Ocular Anatomy, Science, Immunology, Inflammation, Glial Cells, Mouse Models, Research and Analysis Methods, Retinal ganglion, Retina, 03 medical and health sciences, Signs and Symptoms, Model Organisms, Ocular System, medicine, Paralysis, Animals, Optic neuritis, Amnion, Progenitor cell, Cell Proliferation, Multipotent Stem Cells, Biology and Life Sciences, Afferent Neurons, Optic Nerve, Cell Biology, medicine.disease, Mice, Inbred C57BL, Molecular Weight, 030104 developmental biology, Cellular Neuroscience, Animal Studies, Eyes, Myelin-Oligodendrocyte Glycoprotein, Schwann Cells, Clinical Medicine, Peptides, Head, 030217 neurology & neurosurgery, Neuroscience, Demyelinating Diseases |
| الوصف: | ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We compared the effects of ST266 with and without removal of large molecular weight proteins both in vitro and in the multiple sclerosis model experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice. Mice were treated daily with intranasal vehicle, ST266 or lower molecular weight fraction of ST266. Retinal ganglion cells were counted in isolated retinas, and optic nerves were assessed for inflammation and demyelination. ST266 treatment significantly improved retinal ganglion cell survival and reduced optic nerve demyelination in EAE mice. The lower molecular weight ST266 fraction significantly improved optic nerve demyelination, but only showed a trend towards improved retinal ganglion cell survival. ST266 fractions below 50kDa increased Schwann cell proliferation in vitro, but were less effective than non-fractionated ST266. Demyelination attenuation was partially associated with the lower molecular weight ST266 fraction, but removal of higher molecular weight biomolecules from ST266 diminishes its neuroprotective effects, suggesting at least some high molecular weight proteins play a role in ST266-mediated neuroprotection. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ef981f5591411199043aa22cbc6ad98 https://doaj.org/article/ed87775114e3425c94711c78818923c0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3ef981f5591411199043aa22cbc6ad98 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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