Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus)
| العنوان: | Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus) |
|---|---|
| المؤلفون: | Dawn A. Shively, Charles C. Morris, Ashley M. Spoljaric, Katarzyna Przybyla-Kelly, Meredith B. Nevers, Joshua Dickey, Edward F. Roseman, Muruleedhara N. Byappanahalli |
| المصدر: | PLoS ONE, Vol 13, Iss 1, p e0191720 (2018) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, Michigan, Research Facilities, Physiology, Ecological Parameter Monitoring, Marine and Aquatic Sciences, Invasive Species, lcsh:Medicine, Introduced species, Molting, 01 natural sciences, Geographical locations, Food Web Structure, Medicine and Health Sciences, Environmental DNA, Biomass, lcsh:Science, education.field_of_study, Multidisciplinary, biology, Ecology, Goby, Fishes, Eukaryota, Biodiversity, Mesocosms, Freshwater Fish, Community Ecology, Round goby, Forage fish, Vertebrates, Freshwater fish, Research Article, Freshwater Environments, Neogobius, Food Chain, Population, Research and Analysis Methods, 010603 evolutionary biology, Species Colonization, Surface Water, Animals, education, Ecosystem, Population Density, 010604 marine biology & hydrobiology, Ecology and Environmental Sciences, lcsh:R, Organisms, Biology and Life Sciences, Aquatic Environments, DNA, Bodies of Water, biology.organism_classification, United States, Lakes, Fish, North America, Earth Sciences, lcsh:Q, Hydrology, People and places, Introduced Species, Physiological Processes |
| الوصف: | Environmental DNA (eDNA) is revolutionizing biodiversity monitoring, occupancy estimates, and real-time detections of invasive species. In the Great Lakes, the round goby (Neogobius melanostomus), an invasive benthic fish from the Black Sea, has spread to encompass all five lakes and many tributaries, outcompeting or consuming native species; however, estimates of round goby abundance are confounded by behavior and habitat preference, which impact reliable methods for estimating their population. By integrating eDNA into round goby monitoring, improved estimates of biomass may be obtainable. We conducted mesocosm experiments to estimate rates of goby DNA shedding and decay. Further, we compared eDNA with several methods of traditional field sampling to compare its use as an alternative/complementary monitoring method. Environmental DNA decay was comparable to other fish species, and first-order decay was lower at 12°C (k = 0.043) than at 19°C (k = 0.058). Round goby eDNA was routinely detected in known invaded sites of Lake Michigan and its tributaries (range log10 4.8-6.2 CN/L), but not upstream of an artificial fish barrier. Traditional techniques (mark-recapture, seining, trapping) in Lakes Michigan and Huron resulted in fewer, more variable detections than eDNA, but trapping and eDNA were correlated (Pearson R = 0.87). Additional field testing will help correlate round goby abundance with eDNA, providing insight on its role as a prey fish and its impact on food webs. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42026a4fd27338b2fc9c698d537f905a http://europepmc.org/articles/PMC5777661?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....42026a4fd27338b2fc9c698d537f905a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|