The 2-amino group of guanine is absolutely required for specific binding of the anti-cancer antibiotic echinomycin to DNA
| العنوان: | The 2-amino group of guanine is absolutely required for specific binding of the anti-cancer antibiotic echinomycin to DNA |
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| المؤلفون: | Christophe Marchand, Simon E. Moroney, Michael J. Waring, Michael J. McLean, Christian Bailly |
| المصدر: | Nucleic Acids Research. 20:5601-5606 |
| بيانات النشر: | Oxford University Press (OUP), 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Binding Sites, Guanine, Base Sequence, Molecular Sequence Data, Guanosine, DNA, Echinomycin, Biology, Polymerase Chain Reaction, DNA binding site, chemistry.chemical_compound, Quinoxaline, chemistry, CpG site, Biochemistry, Genetics, Deoxyribonuclease I, Binding site |
| الوصف: | The 2-amino group of guanine is believed to be a critical determinant of potential DNA binding sites for echinomycin and related quinoxaline antibiotics. In order to probe its importance directly we have studied the interaction between echinomycin and DNA species in which guanine N(2) is deleted by virtue of substitution of inosine for guanosine residues. The polymerase chain reaction was used to prepare inosine-substituted DNA. Binding of echinomycin, assessed by DNAse I footprinting, was practically abolished by incorporation of inosine into one or both strands of DNA. We conclude that both the purines in the preferred CpG binding site need to bear a 2-amino group to interact with echinomycin. |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::420f6a5e42f34dad79c671303e1a8f31 https://doi.org/10.1093/nar/20.21.5601 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....420f6a5e42f34dad79c671303e1a8f31 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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