Isolation of bovine follicular dendritic cells allows the demonstration of a particular cellular prion protein
| العنوان: | Isolation of bovine follicular dendritic cells allows the demonstration of a particular cellular prion protein |
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| المؤلفون: | F. Leclercq, Ernst Heinen, F. Melot, Y. Frobert, Rikiya Tsunoda, Nadine Antoine, Olivier Jolois, Caroline Thielen |
| المصدر: | Cell and Tissue Research. 306:49-55 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Histology, medicine.drug_class, animal diseases, Palatine Tonsil, Cell Separation, Biology, Monoclonal antibody, Epitope, Pathology and Forensic Medicine, medicine, Animals, PrPC Proteins, Microscopy, Immunoelectron, Follicular dendritic cells, Antibodies, Monoclonal, Germinal center, Cell Biology, Immunogold labelling, Immunohistochemistry, Molecular biology, nervous system diseases, Encephalopathy, Bovine Spongiform, Lymphatic system, biology.protein, Cattle, Lymph Nodes, Lymph, Antibody, Dendritic Cells, Follicular |
| الوصف: | As interaction of cellular prion protein (PrPc) and the infectious agent (PrPres) appears to be a crucial pathogenic step promoted by homology, variation in PrPc isoforms on bovine immune cells may explain the absence of infectivity in most bovine lymph organs. In this study, we examined PrPc expression in bovine lymph organs (tonsils and lymph nodes) and on isolated follicular dendritic cells (FDCs). We used a panel of different monoclonal antibodies (MoAbs) raised against different epitopes of prion protein. Two MoAbs recognise amino acids 79-92 (SAF 34 and SAF 32 Mo-Abs); the 6H4 antibody reacts with a specific peptide comprising the 144-152 amino acids, and the 12F10 MoAb recognises the sequence 142-160. After immunolabelling of frozen sections of lymph organs with 6H4 or 12F10 MoAbs, we detected cellular prion protein in germinal centres. However, using the SAF 34 or SAF 32 antibodies, PrPc was revealed outside the lymphoid tissues. No PrPc was observed in the germinal centres. Therefore, we adapted the method of FDC isolation, making it suitable for the study of PrPc expression on their surface. Using electron microscopy, the presence of PrPc on the surface of FDCs was demonstrated only with 6H4 MoAb. These results suggest that bovine follicular dendritic cells express a particular form of prion protein. Either the N-terminal part of PrPc is cleaved or the accessibility of the specific epitope (79-92) of SAF 34 MoAb is abolished by interaction with other molecules. This particular isoform of PrPc on bovine FDCs might be related to the apparent absence of infectivity in lymph organs in cattle affected by bovine spongiform encephalopathy. |
| تدمد: | 1432-0878 0302-766X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42345e2396da9a9599fe14d0683f3b5d https://doi.org/10.1007/s004410100436 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....42345e2396da9a9599fe14d0683f3b5d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320878 0302766X |
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