Airway remodeling in ferrets with cigarette smoke-induced COPD using µCT imaging
| العنوان: | Airway remodeling in ferrets with cigarette smoke-induced COPD using µCT imaging |
|---|---|
| المؤلفون: | Stephen A. Byzek, Trenton R. Schoeb, Harrison Kim, Hrudaya Nath, Elex S Harris, S. Vamsee Raju, Denise Stanford, Jennifer LaFontaine, Surya P. Bhatt, Steven M. Rowe, Sandeep Bodduluri |
| المصدر: | Am J Physiol Lung Cell Mol Physiol |
| بيانات النشر: | American Physiological Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, COPD, Pathology, medicine.medical_specialty, Chronic bronchitis, Bronchial wall, Physiology, business.industry, Pulmonary disease, Cell Biology, respiratory system, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, 030228 respiratory system, Airway wall, Physiology (medical), medicine, Cigarette smoke, 030212 general & internal medicine, Airway, business, Research Article |
| الوصف: | Structural changes to airway morphology, such as increased bronchial wall thickness (BWT) and airway wall area, are cardinal features of chronic obstructive pulmonary disease (COPD). Ferrets are a recently established animal model uniquely exhibiting similar clinical and pathological characteristics of COPD as humans, including chronic bronchitis. Our objective was to develop a microcomputed tomography (µCT) method for evaluating structural changes to the airways in ferrets and assess whether the effects of smoking induce changes consistent with chronic bronchitis in humans. Ferrets were exposed to mainstream cigarette smoke or air control twice daily for 6 mo. µCT was conducted in vivo at 6 mo; a longitudinal cohort was imaged monthly. Manual measurements of BWT, luminal diameter (LD), and BWT-to-LD ratio (BWT/LD) were conducted and confirmed by a semiautomated algorithm. The square root of bronchial wall area (√WA) versus luminal perimeter was determined on an individual ferret basis. Smoke-exposed ferrets reproducibly demonstrated 34% increased BWT ( P < 0.001) along with increased LD and BWT/LD versus air controls. Regression indicated that the effect of smoking on BWT persisted despite controlling for covariates. Semiautomated measurements replicated findings. √WA for the theoretical median airway luminal perimeter of 4 mm (Pi4) was elevated 4.4% in smoke-exposed ferrets ( P = 0.015). Increased BWT and Pi4 developed steadily over time. µCT-based airway measurements in ferrets are feasible and reproducible. Smoke-exposed ferrets develop increased BWT and Pi4, changes similar to humans with chronic bronchitis. µCT can be used as a significant translational platform to measure dynamic airway morphological changes. |
| تدمد: | 1522-1504 1040-0605 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43d60aac51c1814ce26f8ecce2b3282e https://doi.org/10.1152/ajplung.00328.2019 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....43d60aac51c1814ce26f8ecce2b3282e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
|---|