GENE SILENCING IN FUCUS EMBRYOS: DEVELOPMENTAL CONSEQUENCES OF RNAi-MEDIATED CYTOSKELETAL DISRUPTION
| العنوان: | GENE SILENCING IN FUCUS EMBRYOS: DEVELOPMENTAL CONSEQUENCES OF RNAi-MEDIATED CYTOSKELETAL DISRUPTION |
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| المؤلفون: | Colin Brownlee, Martina Strittmatter, Garry Farnham, Susana M. Coelho, J. M. Cock |
| المساهمون: | Marine Biological Association, Scottish Association for Marine Science (SAMS), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Scottish Association for Marine Science ( SAMS ), Laboratoire de Biologie Intégrative des Modèles Marins ( LBI2M ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Journal of Phycology Journal of Phycology, Wiley, 2013, 49 (5), pp.819-829. ⟨10.1111/jpy.12096⟩ Journal of Phycology, Wiley, 2013, 49 (5), pp.819-829. 〈10.1111/jpy.12096〉 Journal of Phycology, 2013, 49 (5), pp.819-829. ⟨10.1111/jpy.12096⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0106 biological sciences, embryo, Plant Science, Aquatic Science, 01 natural sciences, 03 medical and health sciences, RNA interference, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Gene expression, Botany, Gene silencing, 14. Life underwater, Gene, 030304 developmental biology, 0303 health sciences, Gene knockdown, biology, cytoskeleton, fucus, biology.organism_classification, Cell biology, Brown algae, Multicellular organism, RNA silencing, tubulin, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], RNAi, actin, 010606 plant biology & botany |
| الوصف: | International audience; Brown algae (Phaeophyceae) are an important algal class that play a range of key ecological roles. They are often important components of rocky shore communities. A number of members of the Fucales and Ectocarpales have provided models for the study of multicellular evolution, reproductive biology and polarized development. Indeed the fucoid algae exhibit the unusual feature of inducible embryo polarization, allowing many classical studies of polarity induction. The potential of further studies of brown algae in these important areas has been increasingly hindered by the absence of tools for manipulation of gene expression that would facilitate further mechanistic analysis and gene function studies at a molecular level. The aim of this study was to establish a method that would allow the analysis of gene function through RNAi-mediated gene knockdown. We show that injection of double-stranded RNA (dsRNA) corresponding to an a-tubulin gene into Fucus serratus Linnaeus zygotes induces the loss of a large proportion of the microtubule cytoskeleton, leading to growth arrest and disruption of cell division. Injection of dsRNA targeting b-actin led to reduced rhizoid growth, enlarged cells and the failure to develop apical hair cells. The silencing effect on actin expression was maintained for 3 months. These results indicate that the Fucus embryo possesses a functional RNA interference system that can be exploited to investigate gene function during embryogenesis. |
| اللغة: | English |
| تدمد: | 0022-3646 1529-8817 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45cc76cce3ffdb578a7055b93ac07e71 https://hal.archives-ouvertes.fr/hal-01806438/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....45cc76cce3ffdb578a7055b93ac07e71 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00223646 15298817 |
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